| IED ID | IndEnz0008000005 |
| Enzyme Type ID | cellulase000005 |
| Protein Name |
Endoglucanase 25 EC 3.2.1.4 Cellulase homolog OR16pep Endo-1,4-beta glucanase 25 Protein KORRIGAN Protein RADIALLY SWOLLEN 2 |
| Gene Name | KOR DEC KOR1 RSW2 At5g49720 K2I5.8 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MYGRDPWGGPLEINTADSATDDDRSRNLNDLDRAALSRPLDETQQSWLLGPTEQKKKKYVDLGCIIVSRKIFVWTVGTLVAAALLAGFITLIVKTVPRHHPKTPPPDNYTIALHKALKFFNAQKSGKLPKHNNVSWRGNSGLQDGKGETGSFYKDLVGGYYDAGDAIKFNFPMAYAMTMLSWSVIEYSAKYEAAGELTHVKELIKWGTDYFLKTFNSTADSIDDLVSQVGSGNTDDGNTDPNDHYCWMRPEDMDYKRPVTTCNGGCSDLAAEMAAALASASIVFKDNKEYSKKLVHGAKVVYQFGRTRRGRYSAGTAESSKFYNSSMYWDEFIWGGAWMYYATGNVTYLNLITQPTMAKHAGAFWGGPYYGVFSWDNKLAGAQLLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCSYLPIFNKFNRTNGGLIELNHGAPQPLQYSVNAAFLATLYSDYLDAADTPGWYCGPNFYSTSVLRDFARSQIDYILGKNPRKMSYVVGFGTKYPRHVHHRGASIPKNKVKYNCKGGWKWRDSKKPNPNTIEGAMVAGPDKRDGYRDVRMNYNYTEPTLAGNAGLVAALVALSGEEEATGKIDKNTIFSAVPPLFPTPPPPPAPWKP |
| Enzyme Length | 621 |
| Uniprot Accession Number | Q38890 |
| Absorption | |
| Active Site | ACT_SITE 165; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10140; ACT_SITE 513; /evidence=ECO:0000255|PROSITE-ProRule:PRU10059; ACT_SITE 561; /evidence=ECO:0000255|PROSITE-ProRule:PRU10060; ACT_SITE 570; /evidence=ECO:0000255|PROSITE-ProRule:PRU10060 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; |
| DNA Binding | |
| EC Number | 3.2.1.4 |
| Enzyme Function | FUNCTION: Required for cellulose microfibrils formation. Involved in cell wall assembly during cell elongation and cell plate maturation in cytokinesis. Required for secondary cell wall formation in the developing xylem. May cycle through different intracellular compartments, including plasma membrane. {ECO:0000269|PubMed:10899980, ECO:0000269|PubMed:11351091, ECO:0000269|PubMed:14871312, ECO:0000269|PubMed:16284310, ECO:0000269|PubMed:9755157}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (4); Chain (1); Glycosylation (8); Mutagenesis (7); Region (3); Sequence conflict (6); Topological domain (2); Transmembrane (1) |
| Keywords | Carbohydrate metabolism;Cell membrane;Cell wall biogenesis/degradation;Cellulose degradation;Glycoprotein;Glycosidase;Hydrolase;Membrane;Polysaccharide degradation;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:9755157}; Single-pass type II membrane protein {ECO:0000269|PubMed:9755157}. Note=Cell plate. |
| Modified Residue | |
| Post Translational Modification | PTM: Glycosylated. N-glycosylation of KOR in the endoplasmic reticulum followed by N-glycan modifications in the Golgi are essential for catalytic activity. {ECO:0000269|PubMed:18408158}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10987560; 11169190; 11247609; 11266576; 11289599; 11488474; 12060228; 12492837; 12514238; 16415215; 17317660; 17687051; 18402467; 18583534; 19269997; 19398462; 19704526; 19826767; 22150158; 23148892; 23493405; 23782689; 24728296; 24963054; 25108263; 25238750; 25383767; 25533953; 25535279; 26039485; 26093373; 26896394; 29409894; 30466394; 31334862; 31852774; 32195619; |
| Motif | |
| Gene Encoded By | |
| Mass | 69,191 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |