| IED ID | IndEnz0008000018 |
| Enzyme Type ID | cellulase000018 |
| Protein Name |
Manganese dependent endoglucanase Eg5A EC 3.2.1.4 Carboxymethyl-cellulase 5A CMCase 5A Cellulase 5A Endo-1,4-beta-glucanase Eg5A |
| Gene Name | Eg5A cel5A |
| Organism | Phanerodontia chrysosporium (White-rot fungus) (Sporotrichum pruinosum) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Basidiomycota Agaricomycotina Agaricomycetes Agaricomycetes incertae sedis Polyporales Phanerochaetaceae Phanerodontia Phanerodontia chrysosporium (White-rot fungus) (Sporotrichum pruinosum) |
| Enzyme Sequence | MLKYASIALALATLGVAQQQQWGQCGGIGWTGATTCVAGSVCSVLNPYYSQCIPGAATVTSSSAPSTPTPPAGALPRLGGVNTAGYDFSVATDGSFTGTGVSPPVSQFSHFSSQGANLYRIPFAWQLMTPTLGGTISQSFLSRYDQTVQAALNSGPNVFVIIDLHNYARWNGGIIAQGGPTDAQFQSIWTQLAQKYGSNQRVIFGIMNEPHDIPSISTWVNSVQGAVNAIRAAGATNYLLLPGSSWSSAQAFPTEAGPLLVKVTDPLGGTSKLIFDVHKYLDSDNSGTHPDCTTDNVQVLQTLVQFLQANGNRQAILSETGGGNTSSCESLLANELAYVKSAYPTLAGFSVWAAGAFDTTYVLTVTPNADGSDQPLWVDAVKPNLP |
| Enzyme Length | 386 |
| Uniprot Accession Number | Q66NB7 |
| Absorption | |
| Active Site | ACT_SITE 209; /note=Proton donor; /evidence=ECO:0000305|PubMed:26173955; ACT_SITE 319; /note=Nucleophile; /evidence=ECO:0000305|PubMed:26173955 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; Evidence={ECO:0000269|PubMed:26173955}; |
| DNA Binding | |
| EC Number | 3.2.1.4 |
| Enzyme Function | FUNCTION: Secreted manganese dependent endoglucanase that acts by cleaving the beta-1,4-glucose linkage (PubMed:26173955). Exhibits high activity toward carboxymethyl-cellulose (CMC), barley glucan, and glucomannan (PubMed:26173955). Displays low activity on larminarin and xyloglucan but does not hydrolyze hemicellulose substrates such as birchwood xylan, arabinoxylan, and arabinan (PubMed:26173955). {ECO:0000269|PubMed:26173955}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 50-60 degrees Celsius. {ECO:0000269|PubMed:26173955}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 4.5-6.0. {ECO:0000269|PubMed:26173955}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Domain (1); Glycosylation (1); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Cellulose degradation;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:15888348}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..17; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 40,412 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=3.7 uM for carboxymethyl-cellulose (CMC) {ECO:0000269|PubMed:26173955}; Vmax=208 umol/min/mg enzyme toward carboxymethyl-cellulose (CMC) {ECO:0000269|PubMed:26173955}; |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.2.1.4; |