| IED ID | IndEnz0008000034 |
| Enzyme Type ID | cellulase000034 |
| Protein Name |
Xyloglucan-specific endo-beta-1,4-glucanase BoGH5A EC 3.2.1.151 Glycosyl hydrolase family protein 5A BoGH5A |
| Gene Name | BACOVA_02653 |
| Organism | Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) |
| Taxonomic Lineage | cellular organisms Bacteria FCB group Bacteroidetes/Chlorobi group Bacteroidetes Bacteroidia Bacteroidales Bacteroidaceae Bacteroides Bacteroides ovatus Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) |
| Enzyme Sequence | MEKQSFSDGLFSPLGIKRVIFMLVLLTTSFISCSNSDEKGGSLEVAQEYRNLEFDARGSRQTIQIDGPAEWHISTSESWCKSSHTIGEGKQYVNITVEANDTQKERTATVTVSASGAPDIIINVKQSLYSVPAYDEYIAPDNTGMRDLTSMQLSALMKAGVNVGNTFEAVIVGNDGSLSGDETCWGNPTPNKVLFEGIKAAGFDVVRIPVAYSHQFEDAATYKIKSAWMDKVEAAVKAALDAGLYVIINIHWEGGWLNHPVDANKEALDERLEAMWKQIALRFRDYDDRLLFAGTNEVNNDDANGAQPTEENYRVQNGFNQVFVNTVRATGGRNHYRHLIVQAYNTDVAKAVAHFTMPLDIVQNRIFLECHYYDPYDFTIMPNDENFKSQWGAAFAGGDVSATGQEGDIEATLSSLNVFINNNVPVIIGEYGPTLRDQLTGEALENHLKSRNDYIEYVVKTCVKNKLVPLYWDAGYTEKLFDRTTGQPHNAASIAAIMKGLN |
| Enzyme Length | 502 |
| Uniprot Accession Number | A7LXT7 |
| Absorption | |
| Active Site | ACT_SITE 297; /note=Proton donor; /evidence=ECO:0000250; ACT_SITE 430; /note=Nucleophile; /evidence=ECO:0000305|PubMed:24463512 |
| Activity Regulation | |
| Binding Site | BINDING 165; /note=Substrate; BINDING 172; /note=Substrate; via amide nitrogen; BINDING 251; /note=Substrate; BINDING 296; /note=Substrate; BINDING 472; /note=Substrate |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Xyloglucan + H(2)O = xyloglucan oligosaccharides.; EC=3.2.1.151; Evidence={ECO:0000269|PubMed:24463512}; |
| DNA Binding | |
| EC Number | 3.2.1.151 |
| Enzyme Function | FUNCTION: Catalyzes endohydrolysis of 1,4-beta-D-glucosidic linkages in xyloglucan with retention of the beta-configuration of the glycosyl residues in xyloglucan degradation. Cleaves the backbone of the 3 major types of natural xyloglucans (seed galactoxyloglucan from tamarind kernel, dicot fucogalactoxyloglucan from lettuce leaves, and solanaceous arabinogalactoxyloglucan from tomato fruit), to produce xyloglucan oligosaccharides. {ECO:0000269|PubMed:24463512}. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 6.0-7.0. {ECO:0000269|PubMed:24463512}; |
| Pathway | PATHWAY: Glucan metabolism; xyloglucan degradation. {ECO:0000269|PubMed:24463512}. |
| nucleotide Binding | |
| Features | Active site (2); Beta strand (20); Binding site (5); Chain (1); Domain (1); Helix (15); Lipidation (2); Mutagenesis (2); Signal peptide (1); Turn (3) |
| Keywords | 3D-structure;Carbohydrate metabolism;Cell outer membrane;Glycosidase;Hydrolase;Lipoprotein;Membrane;Palmitate;Polysaccharide degradation;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000305}; Lipid-anchor {ECO:0000305}. Note=Cell outer membrane localization is predicted by analogy with the archetypal sus locus. {ECO:0000269|PubMed:24463512}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..32; /evidence=ECO:0000255|PROSITE-ProRule:PRU00303 |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 3ZMR; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 55,653 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=0.036 mM for XXXG-beta-CNP {ECO:0000269|PubMed:24463512}; KM=0.145 mM for XLLG-beta-CNP {ECO:0000269|PubMed:24463512}; KM=3.59 mM for GGGG-beta-CNP {ECO:0000269|PubMed:24463512}; Note=kcat is 10.5 sec(-1) for XXXG-beta-CNP. kcat is 11.1 sec(-1) for XLLG-beta-CNP. kcat is 0.12 sec(-1) for GGGG-beta-CNP.; |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |