| IED ID | IndEnz0008000039 |
| Enzyme Type ID | cellulase000039 |
| Protein Name |
Exoglucanase A EC 3.2.1.91 1,4-beta-cellobiohydrolase A CBP95 Exocellobiohydrolase A |
| Gene Name | cbhA Celf_1925 |
| Organism | Cellulomonas fimi (strain ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Micrococcales Cellulomonadaceae Cellulomonas Cellulomonas fimi Cellulomonas fimi (strain ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547) |
| Enzyme Sequence | MSTLGKRAGVRRRVRAVATAATATALVAVPLTTLATSASAAPVHVDNPYAGAVQYVNPTWAASVNAAAGRQSADPALAAKMRTVAGQPTAVWMDRISAITGNADGNGLKFHLDNAVAQQKAAGVPLVFNLVIYDLPGRDCFALASNGELPATDAGLARYKSEYIDPIADLLDNPEYESIRIAATIEPDSLPNLTTNISEPACQQAAPYYRQGVKYALDKLHAIPNVYNYIDIGHSGWLGWDSNAGPSATLFAEVAKSTTAGFASIDGFVSDVANTTPLEEPLLSDSSLTINNTPIRSSKFYEWNFDFDEIDYTAHMHRLLVAAGFPSSIGMLVDTSRNGWGGPNRPTSITASTDVNAYVDANRVDRRVHRGAWCNPLGAGIGRFPEATPSGYAASHLDAFVWIKPPGESDGASTDIPNDQGKRFDRMCDPTFVSPKLNNQLTGATPNAPLAGQWFEEQFVTLVKNAYPVIGGTTPVEDLVAPTVPTGLTAGTTTATSVPLSWTASTDNVAVTGYDVYRGTTLVGTTAATSYTVTGLTPATAYSFTVRAKDAAGNVSAASAAAAATTQSGTVTDTTAPSVPAGLTAGTTTTTTVPLSWTASTDNAGGSGVAGYEVLRGTTVVGTTTATSYTVTGLTAGTTYSFSVRAKDVAGNTSAASAAVSATTQTGTVVDTTAPSVPTGLTAGTTTTSSVPLTWTASTDNAGGSGVAGYEVFNGTTRVATVTSTSYTVTGLAADTAYSFTVKAKDVAGNVSAASAAVSARTQAATSGGCTVKYSASSWNTGFTGTVEVKNNGTAALNGWTLGFSFADGQKVSQGWSAEWSQSGTAVTAKNAPWNGTLAAGSSVSIGFNGTHNGTNTAPTAFTLNGVACTLG |
| Enzyme Length | 872 |
| Uniprot Accession Number | P50401 |
| Absorption | |
| Active Site | ACT_SITE 188; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10057; ACT_SITE 410; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10056 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.; EC=3.2.1.91; |
| DNA Binding | |
| EC Number | 3.2.1.91 |
| Enzyme Function | FUNCTION: This enzyme hydrolyzes 1,4-beta-D-glucosidic linkages of cellulose. Weak activity against carboxymethylcellulose, bacterial microcrystalline cellulose and barley beta-glucan. Has also weak endoglucanase activity. Hydrolyzes glucosidic bonds with inversion of anomeric configuration. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Beta strand (12); Chain (1); Disulfide bond (3); Domain (4); Helix (19); Region (1); Signal peptide (1); Turn (4) |
| Keywords | 3D-structure;Carbohydrate metabolism;Cellulose degradation;Direct protein sequencing;Disulfide bond;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Repeat;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..40; /evidence=ECO:0000269|PubMed:8458833 |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 7CBD; |
| Mapped Pubmed ID | 32816991; |
| Motif | |
| Gene Encoded By | |
| Mass | 89,301 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |