| IED ID | IndEnz0008000040 |
| Enzyme Type ID | cellulase000040 |
| Protein Name |
Exoglucanase B EC 3.2.1.91 1,4-beta-cellobiohydrolase B CBP120 Exocellobiohydrolase B |
| Gene Name | cbhB cenE Celf_3400 |
| Organism | Cellulomonas fimi (strain ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Micrococcales Cellulomonadaceae Cellulomonas Cellulomonas fimi Cellulomonas fimi (strain ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547) |
| Enzyme Sequence | MSSTTRRRSAWVAAATVGVSSFLAVAGITPAIAAAGAGQPATVTVPAASPVRAAVDGEYAQRFLAQYDKIKDPANGYFSAQGIPYHAVETLMVEAPDYGHETTSEAYSYWLWLEALYGQVTQDWAPLNHAWDTMEKYMIPQSVDQPTNSFYNPNSPATYAPEFNHPSSYPSQLNSGISGGTDPIGAELKATYGNADVYQMHWLADVDNIYGFGATPGAGCTLGPTATGTSFINTFQRGPQESVWETVPQPSCEEFKYGGKNGYLDLFTKDASYAKQWKYTSASDADARAVEAVYWANQWATEQGKAADVAATVAKAAKMGDYLRYTLFDKYFKKIGCTSPTCAAGQGREAAHYLLSWYMAWGGATDTSSGWAWRIGSSHAHFGYQNPLAAWALSTDPKLTPKSPTAKADWAASMQRQLEFYTWLQASNGGIAGGATNSWDGAYAQPPAGTPTFYGMGYTEAPVYVDPPSNRWFGMQAWGVQRVAELYYASGNAQAKKILDKWVPWVVANISTDGASWKVPSELKWTGKPDTWNAAAPTGNPGLTVEVTSYGQDVGVAADTARALLFYAAKSGDTASRDKAKALLDAIWANNQDPLGVSAVETRGDYKRFDDTYVANGDGIYIPSGWTGTMPNGDVIKPGVSFLDIRSFYKKDPNWSKVQTFLDGGAEPQFRYHRFWAQTAVAGALADYARLFDDGTTTPDTTAPTVPTGLQAGVVTSTEATISWTASTDDTRVTGYDVYRGATKVGTATTTSFTDTGLTASTAYAYTVRAFDAAGNVSAPSAALTVTTKATPSDTTAPSVPAITSSSSTANSVTIGWSASTDNAGGSGLAGYDVYRGATRVAQTTALTFTDTGLTASTAYEYTVRARDVAGNVSAPSTAVSVTTKSDTTPDTTAPSVPAGLAAMTVTETSVALTWNASTDTGGSGLKGYDVYRGATRVGSTTTASYTDTGLTAATAYQYTVRATDNAGNVSAASAALSVTTKTPQTGGSCSVAYNASSWNSGFTASVRITNTGTTTINGWSLGFDLTAGQKVQQGWSATWTQSGSTVTATNAPWNGTLAPGQTVDVGFNGSHTGQNPNPASFTLNGASCT |
| Enzyme Length | 1090 |
| Uniprot Accession Number | P50899 |
| Absorption | |
| Active Site | ACT_SITE 513; /note=Nucleophile; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.; EC=3.2.1.91; |
| DNA Binding | |
| EC Number | 3.2.1.91 |
| Enzyme Function | FUNCTION: Hydrolyzes cellohexaose to a mixture of cellotetraose, cellotriose and cellobiose, with only a trace of glucose. It hydrolyzed cellopentaose to cellotriose and cellobiose, and cellotetraose to cellobiose, but it did not hydrolyze cellotriose. Has also weak endoglucanase activity. Hydrolyzes glucosidic bonds with inversion of anomeric configuration. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Disulfide bond (1); Domain (4); Propeptide (1); Region (2); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Cellulose degradation;Direct protein sequencing;Disulfide bond;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Repeat;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..33; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 114,829 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |