| IED ID | IndEnz0008000045 |
| Enzyme Type ID | cellulase000045 |
| Protein Name |
Glucosylceramidase EC 3.2.1.45 Endoglycoceramidase-related protein 1 EGCrP1 Glucocerebrosidase |
| Gene Name | EGC1 CNAG_00623 |
| Organism | Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Basidiomycota Agaricomycotina Tremellomycetes Tremellales (jelly fungi) Cryptococcaceae Cryptococcus Cryptococcus neoformans species complex Cryptococcus neoformans (Filobasidiella neoformans) Cryptococcus neoformans var. grubii (Filobasidiella neoformans var. grubii) Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii) |
| Enzyme Sequence | MSGIQFDVSRLCAATISIQGRHFVDSHGRVLHLRGANVSAASKVPATPAPKIHDHAQASYVGRPFRLEEADEHWARLKSWGLTFVRITVTWEALEHKERGVYDEDYLAYLRALLQSMEPYGLVAYIALHQDVWSRYCGGSGAPGWTLEAAGFDLSNEGENLSLSGAAFLDGIKSGRLAGERGLWPTGYQKLAAATMNTLFWGGETFAPLLKVPGQIDGKWVSRNIQVYLQEAFLAATAKLVKAVGDLETVMGFELMNEPHPGFIGIQSIHEWDYTTDLHLGQFPSPLQSFSMGAGHPTPNVPVYTRSFPFPTRVTSHVTANPEGACAWASKECPWEKHGVWRWSEAKQEAAALQQDYFVKNRDGGKVDFYEDFYFPFVRKWEQVIGENISSTKGLKARMVEAIPNELCPEWKEESRPKNMVYAPHWYDLNTLFKKKFGFMSVNVQGLARGMFILRALYFGTAAAKANYALQIKTIVLAARLKLGPVPVIFGECGVPMDINNEEAFRTGDWKWQERSMDALISAMEGALMGFNLWTYNPANRDDIGDDWNAENFSWYSESNRTKLLKNAEKSSDGLDVGARLLNVIVRPYPIATAGNPTSLAYDANACAFTYRFRSPLRVSAAAPTPEEYTEIFLPRRVFRKESTEWTVTAGGKVHVDWERERVFVWFEDSSLTAASIKDDTRPRRIDIWVIGRKVEENWSIAQILVAVVILLLGVLVAYYAQLYEWEKDKMIFQHLREANGM |
| Enzyme Length | 742 |
| Uniprot Accession Number | H1AE12 |
| Absorption | |
| Active Site | ACT_SITE 258; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:O85465; ACT_SITE 492; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:O85465 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose; Xref=Rhea:RHEA:13269, ChEBI:CHEBI:4167, ChEBI:CHEBI:15377, ChEBI:CHEBI:22801, ChEBI:CHEBI:52639; EC=3.2.1.45; Evidence={ECO:0000269|PubMed:22072709};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:13270; Evidence={ECO:0000269|PubMed:22072709}; |
| DNA Binding | |
| EC Number | 3.2.1.45 |
| Enzyme Function | FUNCTION: Specifically hydrolyzes the glucosidic linkage in glucosylceramide (PubMed:22072709). May prevent accumulation of aberrent glucosylceramide containing immature ceramide (PubMed:22072709). {ECO:0000269|PubMed:22072709}. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7. {ECO:0000269|PubMed:22072709}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Erroneous initiation (1); Glycosylation (6); Transmembrane (1) |
| Keywords | Glycoprotein;Glycosidase;Hydrolase;Membrane;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Membrane {ECO:0000269|PubMed:22072709}; Single-pass membrane protein {ECO:0000255}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 83,552 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:13269; RHEA:13270 |
| Cross Reference Brenda | 3.2.1.62; |