| IED ID | IndEnz0008000048 |
| Enzyme Type ID | cellulase000048 |
| Protein Name |
Endoglucanase 1 EC 3.2.1.4 Cellulase 1 Endo-1,4-beta-glucanase 1 |
| Gene Name | egl1 MGG_02532 |
| Organism | Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Sordariomycetidae Magnaporthales Pyriculariaceae Pyricularia Magnaporthe oryzae (Rice blast fungus) (Pyricularia oryzae) Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae) |
| Enzyme Sequence | MAKFSALCSLALLGLATAQKPVGTETHEKLTTFRCTVAGGCVEKTNYIVLDSITGHRIYQPAAETLDCGARGAAPNVTACPTKEACAENCAMEGRTDYGSQGVSTDGASLRLQILHDGKKVAPRVYLLDETEAKYEMLRLTGNEFAFEVTMDKLPCGMNSALYLSEMEEDGGKSELNPGGAPWGTGYCDAQCYVTPFINGEGNIKGNGACCAEMDIWEANSRATHIAPHPCSKPGLYLCEGDECGSTGVCDKSGCAWNPNRIAQPHYYGNNDTFKVDTLKPMTVVTQFPTDASGKLAAIRRLYVQGGVVIKAETVHKAGLPEVDALTDPFCEAFGSQRYMALGATGGMGDALARGMVLVMSIWWDETGGNMQWLDGIASGSGPCNATEGAPANIPLVEPNPEVTFSNLKWGEIGSTFQGGARRL |
| Enzyme Length | 424 |
| Uniprot Accession Number | G4NK46 |
| Absorption | |
| Active Site | ACT_SITE 213; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:P56680; ACT_SITE 218; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:P56680 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; Evidence={ECO:0000269|PubMed:18247030}; |
| DNA Binding | |
| EC Number | 3.2.1.4 |
| Enzyme Function | FUNCTION: Endoglucanase that is involved in the biological conversion of cellulose to glucose. Hydrolyses internal beta-1,4-glucosidic bonds. {ECO:0000269|PubMed:18247030}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Disulfide bond (9); Glycosylation (3); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Cellulose degradation;Disulfide bond;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | INDUCTION: Expression is increased at 72 h of infection, and then the expression level drops back to the lower level for the remainder of the time course. {ECO:0000269|PubMed:18247030}. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:18247030}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..18; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 45,062 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |