| IED ID | IndEnz0008000064 |
| Enzyme Type ID | cellulase000064 |
| Protein Name |
Endo-1,6-beta-glucanase EC 3.2.1.75 Fragment |
| Gene Name | |
| Organism | Acremonium sp. |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Hypocreomycetidae Hypocreales Hypocreales incertae sedis Acremonium unclassified Acremonium Acremonium sp. |
| Enzyme Sequence | WLSGVDKIRGVNLGSAFIIE |
| Enzyme Length | 20 |
| Uniprot Accession Number | P82288 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random hydrolysis of (1->6)-linkages in (1->6)-beta-D-glucans.; EC=3.2.1.75; Evidence={ECO:0000269|Ref.1}; |
| DNA Binding | |
| EC Number | 3.2.1.75 |
| Enzyme Function | FUNCTION: Endo-1,6-beta-glucanase that has highest activity against the beta-1,6-glucan pustulan. Also active against the beta-1,6-glucan lutean. Lower activity against laminarin (beta-1,3-glucan with beta-1,6-branches). Little or no activity against gentiobiose, yeast glucan, lichenin, scleroglucan, curdlan, barley glucan, CM cellulose, HE cellulose, pachyman and pullulan. {ECO:0000269|Ref.1}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 50 degrees Celsius at pH 5.0. Stable when incubated for 30 minutes at up to 50 degrees Celsius, little activity at 75 degrees Celsius or above. {ECO:0000269|Ref.1}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 6.5 at 40 degrees Celsius. Retains at least 80% of activity after incubation for 24 hours at 4 degrees Celsius between pH 4.5 and pH 9.5. {ECO:0000269|Ref.1}; |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Non-terminal residue (1) |
| Keywords | Cell wall biogenesis/degradation;Direct protein sequencing;Glycosidase;Hydrolase;Secreted |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, extracellular space {ECO:0000269|Ref.1}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 2,175 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.2.1.75; |