Detail Information for IndEnz0008000064
IED ID IndEnz0008000064
Enzyme Type ID cellulase000064
Protein Name Endo-1,6-beta-glucanase
EC 3.2.1.75
Fragment
Gene Name
Organism Acremonium sp.
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Hypocreomycetidae Hypocreales Hypocreales incertae sedis Acremonium unclassified Acremonium Acremonium sp.
Enzyme Sequence WLSGVDKIRGVNLGSAFIIE
Enzyme Length 20
Uniprot Accession Number P82288
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Random hydrolysis of (1->6)-linkages in (1->6)-beta-D-glucans.; EC=3.2.1.75; Evidence={ECO:0000269|Ref.1};
DNA Binding
EC Number 3.2.1.75
Enzyme Function FUNCTION: Endo-1,6-beta-glucanase that has highest activity against the beta-1,6-glucan pustulan. Also active against the beta-1,6-glucan lutean. Lower activity against laminarin (beta-1,3-glucan with beta-1,6-branches). Little or no activity against gentiobiose, yeast glucan, lichenin, scleroglucan, curdlan, barley glucan, CM cellulose, HE cellulose, pachyman and pullulan. {ECO:0000269|Ref.1}.
Temperature Dependency BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 50 degrees Celsius at pH 5.0. Stable when incubated for 30 minutes at up to 50 degrees Celsius, little activity at 75 degrees Celsius or above. {ECO:0000269|Ref.1};
PH Dependency BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 6.5 at 40 degrees Celsius. Retains at least 80% of activity after incubation for 24 hours at 4 degrees Celsius between pH 4.5 and pH 9.5. {ECO:0000269|Ref.1};
Pathway
nucleotide Binding
Features Chain (1); Non-terminal residue (1)
Keywords Cell wall biogenesis/degradation;Direct protein sequencing;Glycosidase;Hydrolase;Secreted
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted, extracellular space {ECO:0000269|Ref.1}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 2,175
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda 3.2.1.75;