| IED ID | IndEnz0008000082 |
| Enzyme Type ID | cellulase000082 |
| Protein Name |
Mannan endo-1,4-beta-mannosidase 5 EC 3.2.1.78 Beta-mannanase 5 Endo-beta-1,4-mannanase 5 AtMAN5 |
| Gene Name | MAN5 At4g28320 F26K10.200 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MVPTRNRPMLRILGFFICAAFIYLSFRDLWLNHKGKAKLGFVKRNGTQFVVDDKPLYVNGWNSYWFMDHAVDEHSRNLVGEMLEAGAKMGLTVCRTWAFNDGGYNALQISPGRFDERVFQALDHVIAEARKHDVRLLLSLVNNLQAYGGKTQYVKWAWQEGVGLSSSNDSFFFDPSIRNYFKNYLKVLLTRKNSVTGIEYRNDPTIFAWELINEPRCTTDVSGKTLQDWIDEMTGFIKSIDDKHLLTVGLEGFYGPNSPKGLTVNPEQWASQLGTDFVQNSNSSNIDFASVHIYPDHWFHNQTFEEKLKFVVKWMQSHIEDGLKELKKPVLFTEFGLSNQNKDYEPSQRDKFYRIIFDVVYKSAKRKKSGAGTLVWQLFMEGMETFNDDFGIVPHEQDSIYKLMIEQSCRLGKVTGRLKEQKLKELCSHRH |
| Enzyme Length | 431 |
| Uniprot Accession Number | Q9M0H6 |
| Absorption | |
| Active Site | ACT_SITE 214; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:Q99036; ACT_SITE 334; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q99036 |
| Activity Regulation | |
| Binding Site | BINDING 97; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 213; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 294; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 376; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.; EC=3.2.1.78; |
| DNA Binding | |
| EC Number | 3.2.1.78 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Binding site (4); Chain (1); Glycosylation (4); Signal peptide (1) |
| Keywords | Glycoprotein;Glycosidase;Hydrolase;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..24; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 20878180; 21301215; 31266899; |
| Motif | |
| Gene Encoded By | |
| Mass | 49,899 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.2.1.78; |