| IED ID | IndEnz0008000085 |
| Enzyme Type ID | cellulase000085 |
| Protein Name |
Mannan endo-1,4-beta-mannosidase 3 EC 3.2.1.78 Beta-mannanase 3 Endo-beta-1,4-mannanase 3 OsMAN3 |
| Gene Name | MAN3 Os03g0828300 LOC_Os03g61270 OSJNBa0010E04.20 |
| Organism | Oryza sativa subsp. japonica (Rice) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Oryzoideae Oryzeae Oryzinae Oryza Oryza sativa (Rice) Oryza sativa subsp. japonica (Rice) |
| Enzyme Sequence | MTVRPRPAAAAIIIAAVFGAAAAAAGGGMVGVDGTQFVVEGGRTIYFSGFNAYWLMMMASDPARRAAVVAAFAQASSRGLNLARTWAFSDGGDQPLQSSPGVYDEAMFQGLDFVIAEARRHGIYLLLCLTNNFDDFGGKRQYVRWAADAGHNLTAGDDFFTSSVVKSYYKNHVKAVLTRVNTVTGVAYKDDPTIFAWELMNEPRCDADPTGGMVQAWVEEMAPYVKRVDGGRHLVTAGLEGFYGDGEHESKELNPWGIYYGTNYVATHRAAGVDFATIHLYPDVWLWGSTADEQAAFFRNWTRSHVHDTAAFLGKPLLVTEYGKFLWKGGGANKTQRNYFLDVVLDAIYASASRGGPLVGGAFWQLLLDDDVVAGMDDLRDGYEIILAEDSRAASIIGEHSEQLASLNGQDAEALRRRRRRPASSHRKTRLGSGGDSDALRLPRTLLIRFISLSRSISSFIQDNFVLF |
| Enzyme Length | 468 |
| Uniprot Accession Number | Q0DM48 |
| Absorption | |
| Active Site | ACT_SITE 202; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:Q99036; ACT_SITE 321; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q99036 |
| Activity Regulation | |
| Binding Site | BINDING 86; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 201; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 281; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 364; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 371; /note=Substrate; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:B4XC07 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.; EC=3.2.1.78; |
| DNA Binding | |
| EC Number | 3.2.1.78 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Binding site (5); Chain (1); Erroneous gene model prediction (2); Glycosylation (3); Region (1); Signal peptide (1) |
| Keywords | Glycoprotein;Glycosidase;Hydrolase;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..23; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 51,217 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |