| IED ID | IndEnz0008000087 |
| Enzyme Type ID | cellulase000087 |
| Protein Name |
Mannan endo-1,4-beta-mannosidase 7 EC 3.2.1.78 Beta-mannanase 7 Endo-beta-1,4-mannanase 7 AtMAN7 |
| Gene Name | MAN7 At5g66460 K1F13.12 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MKLLALFPFLAIVIQLSCWELGTDALPSGGFVRTKGVQFSLNGYPYYANGFNAYWLMYVASDPSQRSKISTAFQDASRHGLTVARTWAFSDGGYRALQYSPGSYNEDMFQGLDFALAEARRHGIKIILSFANNYESFGGRKQYVDWARSRGRPVSSEDDFFTDSLVKDFYKNHIKAVLNRFNTFTKVHYKDDPTIMAWELMNEPRCPSDPSGRAIQAWITEMAAHVKSLDRNHLLEAGLEGFYGQSSPQSKTLNPPGQFGTDFIANNRIPGIDFVTVHSYPDEWFPDSSEQSQMDFLNKWLDAHIQDAQNVLHKPIILAEFGKSMKKPGYTPAQRDIVFNTVYSKIYGSAKRGGAAAGGLFWQLLVNGIDNFQDGYGIILSQSSSTVNVISQQSRKLTLIRKIFARMINVEKWKRARGQGQVGKRGHKINN |
| Enzyme Length | 431 |
| Uniprot Accession Number | Q9FJZ3 |
| Absorption | |
| Active Site | ACT_SITE 203; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:B3A0S5; ACT_SITE 320; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:B3A0S5 |
| Activity Regulation | |
| Binding Site | BINDING 87; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 202; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 280; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 362; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.; EC=3.2.1.78; |
| DNA Binding | |
| EC Number | 3.2.1.78 |
| Enzyme Function | FUNCTION: Required for both, loosening of the micropylar endosperm, and rupture of the seed coat in germinating seeds. May participate in the hydrolysis of the mannans in the cell wall of germinating seeds. {ECO:0000269|PubMed:23461773}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Binding site (4); Chain (1); Signal peptide (1) |
| Keywords | Glycosidase;Hydrolase;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | INDUCTION: By gibberellin in germinating seeds. {ECO:0000269|PubMed:23461773}. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:23461773, ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..25; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 16615871; 16920880; 18359847; 20878180; 21301215; 21798944; 29348141; |
| Motif | |
| Gene Encoded By | |
| Mass | 48,573 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.2.1.78; |