| IED ID | IndEnz0008000090 |
| Enzyme Type ID | cellulase000090 |
| Protein Name |
Probable mannan endo-1,4-beta-mannosidase A-2 EC 3.2.1.78 Endo-beta-1,4-mannanase A-2 |
| Gene Name | manA-2 man1-2 ATEG_02669 |
| Organism | Aspergillus terreus (strain NIH 2624 / FGSC A1156) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Circumdati Aspergillus terreus Aspergillus terreus (strain NIH 2624 / FGSC A1156) |
| Enzyme Sequence | MKVPRLLLALGGLASIHIASALPQGAVERDMHATSAVVHGGNVKTKFPSTTGLKFTIDGETGYFAGSNSYWIGFLTNNADVDLVFQHMKESGLKILRVWGFNDVNTKPATGTVWYQLHANGTSTINTGPDGLQRLDYVVHSAERHGIKLIINFVNNWNDYGGINSYLQAYGGASNEDFYNSEPMQKAYRAYIKEVVSRYIDSPAVFAWELANEPRCKGCDTSVLHSWIEKTSRYIKSLDKKHMVTTGEEGFGLDLMSDGSYPFTYVEGGNFTDTLSIPTIDFGTFHLYPSSWGTTNDWGNLWVEAHGAACKAAGKPRLFEEYGVTADHCALEKPWQTTALKTKGVSGDLYWQYGDTLSTGPSPDDGNTFYYGTDEFDCLVSDHVAAIDEMEKHGRH |
| Enzyme Length | 396 |
| Uniprot Accession Number | Q0CUG5 |
| Absorption | |
| Active Site | ACT_SITE 213; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:Q99036; ACT_SITE 321; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q99036 |
| Activity Regulation | |
| Binding Site | BINDING 99; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 212; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 288; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 351; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.; EC=3.2.1.78; |
| DNA Binding | |
| EC Number | 3.2.1.78 |
| Enzyme Function | FUNCTION: Endo-1,4-mannanase, a crucial enzyme for depolymerization of seed galactomannans and wood galactoglucomannans. {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Binding site (4); Chain (1); Erroneous initiation (1); Glycosylation (2); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Glycoprotein;Glycosidase;Hydrolase;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..21; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 43,756 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |