| IED ID | IndEnz0008000110 |
| Enzyme Type ID | cellulase000110 |
| Protein Name |
Endoglucanase 1 EC 3.2.1.4 CEL1 CMCase I Cellulase I Endo-1,4-beta-glucanase 1 |
| Gene Name | celA1 |
| Organism | Streptomyces halstedii |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Streptomycetales Streptomycetaceae Streptomyces Streptomyces griseus group Streptomyces halstedii subgroup Streptomyces halstedii |
| Enzyme Sequence | MSRKLRTLMAALCALPLAFAAAPPAHAADPTTMTNGFYADPDSSASRWAAANPGDGRAAAINASIANTPMARWFGSWSGAIGTAAGAYAGAADGRDKLPILVAYNIYNRDYCGGHSAGGAASPSAYADWIARFAGGIAARPAVVILEPDSLGDYGCMNPAQIDEREAMLTNALVQFNRQAPNTWVYMDAGNPRWADAATMARRLHEAGLRQAHGFSLNVSNYITTAENTAYGNAVNNELAARYGYTKPFVVDTSRNGNGSNGEWCNPSGRRIGTPTRTGGGAEMLLWIKTPGESDGNCGVGSGSTAGQFLPEVAYKMIYGY |
| Enzyme Length | 321 |
| Uniprot Accession Number | P33682 |
| Absorption | |
| Active Site | ACT_SITE 110; /evidence=ECO:0000250; ACT_SITE 149; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10057; ACT_SITE 295; /note=Nucleophile; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; |
| DNA Binding | |
| EC Number | 3.2.1.4 |
| Enzyme Function | FUNCTION: Implicated in the mechanism of induction exerted by cellobiose. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (1); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Cellulose degradation;Direct protein sequencing;Disulfide bond;Glycosidase;Hydrolase;Polysaccharide degradation;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..27; /evidence=ECO:0000269|PubMed:1400190 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 33,695 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |