IED ID | IndEnz0008000111 |
Enzyme Type ID | cellulase000111 |
Protein Name |
Cellulase 1 EC 3.2.1.4 Avicelase Endo-1,4-beta-glucanase Endoglucanase |
Gene Name | cel1 |
Organism | Streptomyces reticuli |
Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Streptomycetales Streptomycetaceae Streptomyces Streptomyces reticuli |
Enzyme Sequence | MKRRTTAVLTLTALLGTALTALPVQQAGAEEVEQVRNGTFDTTTDPWWTSNVTAGLSDGRLCADVPGGTTNRWDSAIGQNDITLVKGETYRFSFHASGIPEGHVVRAVVGLAVSPYDTWQEASPVLTEADGSYSYTFTAPVDTTQGQVAFQVGGSTDAWRFCVDDVSLLGGVPPEVYEPDTGPRVRVNQVAYLPAGPKNATLVTDATARLPWQLRNAQGTTVARGLTVPRGVDASSGQNVHSIDFGSYRGRGTGYTLVADGETSHPFDIDAAAYRPLRLDSVKYYYTQRSGIAIRDDLRPGYGRAAGHLNVAPNQGDANVPCQPGVCDYTLDVTGGWYDAGDHGKYVVNGGIATWELLSTYERSLTARTGHPAALGDGTLALPESGNKVPDVLDEARWELEFLLKMQVPAGQPLAGMAHHKLHDEQWTGLPLLPDQDPQKRELHPPTTAATLNLAATAAQAARLYRPFDKAFAARALTAARTAWQAALAHPDLLADPNDGTGGGAYNDDDVTDEFYWAAAELYLTTGERQFADHVLDSPVHTADIFGPTGFDWGHTAAAGRLDLALVPSRLPGRDQVRRSVIKAADTYLATLTAHPYGMPYAPAGNRYDWGSSHQVLNNGVVLASAYDLTGAAKYRDGALQGMDYVLGRNALNMSYVTGYGEVSSHNQHSRWYAHQLDPTLPNPPSGTLAGGPNSSIQDPYAQSKLTGCVGQFCYIDDIQSWSTNETAINWNAALARMASFAADQG |
Enzyme Length | 746 |
Uniprot Accession Number | Q05156 |
Absorption | |
Active Site | ACT_SITE 342; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10140; ACT_SITE 669; /evidence=ECO:0000255|PROSITE-ProRule:PRU10059; ACT_SITE 717; /evidence=ECO:0000255|PROSITE-ProRule:PRU10060; ACT_SITE 726; /evidence=ECO:0000255|PROSITE-ProRule:PRU10060 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; |
DNA Binding | |
EC Number | 3.2.1.4 |
Enzyme Function | FUNCTION: This endoglucanase acts only on crystalline cellulose. |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Active site (4); Chain (1); Domain (1); Region (2); Signal peptide (1) |
Keywords | Carbohydrate metabolism;Cellulose degradation;Direct protein sequencing;Glycosidase;Hydrolase;Polysaccharide degradation;Signal |
Interact With | |
Induction | |
Subcellular Location | SUBCELLULAR LOCATION: Note=Exists in both mycelium-associated and extracellular forms. |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | SIGNAL 1..29; /evidence=ECO:0000269|PubMed:1282194 |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 80,000 |
Kinetics | |
Metal Binding | |
Rhea ID | |
Cross Reference Brenda |