| IED ID | IndEnz0008000122 |
| Enzyme Type ID | cellulase000122 |
| Protein Name |
Mannan endo-1,4-beta-mannosidase EC 3.2.1.78 Mannanase 5A Man5A Mannanase A ManA |
| Gene Name | man5A CJA_3338 |
| Organism | Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Cellvibrionales Cellvibrionaceae Cellvibrio Cellvibrio japonicus (Pseudomonas fluorescens subsp. cellulosa) Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa) |
| Enzyme Sequence | MSLFTPLSETNVRSHTNTSSVFCRRIKTLVAGLTALGLMLAAVSASAGFYVSGKQLREGNGNNFIMRGVNLPHAWFPDRTNQALADISATGANSVRVVLSNGRLWSRTPESQVASIISQAKARQLITVLEVHDTTGYGEQTAATLSEAVDYWIAIRNALIGQEDYVIINIGNEPFGNGQSASTWLNLHRDAINRLRNAGFTHTLMVDAANWGQDWENIMRNNASSLFNSDPRRNVIFSVHMYEVYPNDTAVNNYMSAFNSMNLPLVVGEFAANHFGSYVDAGSIMARAQQYGFGYLGWSWSGNSSNLSALDVVTNFNAGSLTTWGNLLINNTNGIRNTSRKATIFGGSGSSSSSAGSCGTAPNGYPYCCNASSATGNGWGWENNRSCVVATTSTSCNWYGTSYPICVNTSSGWGWENNRSCIAASTCAAQ |
| Enzyme Length | 430 |
| Uniprot Accession Number | B3PF24 |
| Absorption | |
| Active Site | ACT_SITE 173; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:P22533; ACT_SITE 269; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:P22533 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.; EC=3.2.1.78; Evidence={ECO:0000269|PubMed:19441796}; |
| DNA Binding | |
| EC Number | 3.2.1.78 |
| Enzyme Function | FUNCTION: Catalyzes the endo hydrolysis of beta-1,4-linked mannan, galactomannan and glucomannan. It is able to hydrolyze mannosidic linkages that are flanked by mannose or glucose. {ECO:0000269|PubMed:19441796}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Domain (2) |
| Keywords | Glycosidase;Hydrolase;Reference proteome;Repeat |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 46,325 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=6.2 mg/ml for glucomannan {ECO:0000269|PubMed:19441796}; KM=8.5 mg/ml for galactomannan {ECO:0000269|PubMed:19441796}; Note=kcat is 140000 min(-1) for mannanase activity with galactomannan as substrate. kcat is 62000 min(-1) for mannanase activity with glucomannan as substrate. {ECO:0000269|PubMed:19441796}; |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |