| IED ID | IndEnz0008000124 |
| Enzyme Type ID | cellulase000124 |
| Protein Name |
Putative mannan endo-1,4-beta-mannosidase 5 EC 3.2.1.78 Beta-mannanase 5 Endo-beta-1,4-mannanase 5 OsMAN5 |
| Gene Name | MAN5 Os05g0319100 LOC_Os05g25480 OSJNBb0006B22.2 OSJNBb0059K16.9 |
| Organism | Oryza sativa subsp. japonica (Rice) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Oryzoideae Oryzeae Oryzinae Oryza Oryza sativa (Rice) Oryza sativa subsp. japonica (Rice) |
| Enzyme Sequence | METSYREEEARRKASLLHCIFFFLLGALAMAAAIAVLHESSYWEWRCNRLTDIVVDGDDGDGPSSSEVVDGGGEWGMVRTRGAQFVVGGGRPFYVNGFNTYWLMVLAVDPSTRGKVTEVFRQAAAVGLTVCRTWAFNDGGWRALQKSPGVYDEEVFKALDFVVSEARKHKIRLILPLINNWDDYGGKAQYVRWAQAAAAGAGADAFFSDETVRGYFKSHVTAVLTRVNAYTGVAYRDDPTIMAWELMNEPRCASDPTGDTLQAWIAEMAFHVKSVDPAHLLGVGAEGFYGPSSPPARLRVNPNADVALAGADFVRNHRVLGVDFASVHVYPDTWLPAGATKEAQLRFATSWVEAHIADAEGALGGMPVLFAEFGVSTRGARAAFNATSRDAFIEAVYGAMLRSTRRGGGGAGALLWQVFPEGTDYMDDGYAVVLPRAAATAGIVAAHSRRLQSFNSRCAWSCRWGCNKRDNDTAETTTAEADVDVSFHHEL |
| Enzyme Length | 491 |
| Uniprot Accession Number | Q5W6G0 |
| Absorption | |
| Active Site | ACT_SITE 249; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:Q99036; ACT_SITE 372; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q99036 |
| Activity Regulation | |
| Binding Site | BINDING 134; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 248; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 330; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 416; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.; EC=3.2.1.78; |
| DNA Binding | |
| EC Number | 3.2.1.78 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Binding site (4); Chain (1); Erroneous initiation (2); Glycosylation (2); Signal peptide (1) |
| Keywords | Glycoprotein;Glycosidase;Hydrolase;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..31; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 53,465 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |