IED ID | IndEnz0008000125 |
Enzyme Type ID | cellulase000125 |
Protein Name |
Probable mannan endo-1,4-beta-mannosidase A EC 3.2.1.78 Endo-beta-1,4-mannanase A |
Gene Name | manA man1 AFLA_116950 |
Organism | Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167) |
Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Circumdati Aspergillus flavus Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167) |
Enzyme Sequence | MKLNPSLLTAAGLVSAQLASALPQASSSTVSPSPSPSATPGSFVTTSGLNFVIDGKTGYFAGSNSYWIGFQKNNDDVDLVFSHLQESGLKILRVWGFNDVNQKPTDGSVYYHLLADGTATVNEGEDGLQRLDYVVSSAEKHGIKLIINFVNFWDDYGGINAYVKAFGGSKEDFYTNDAMQAAYRAYIKAVISRYSDSTAIFAWELANEPRCQGCETTVLYNWIESTSQYIKSLDSKHLVCIGDEGFGLDTGSDGSYPYQYSEGSDFAKNLAIPTIDFGTFHLYPSSWGTTNDWGNGWVTSHGAACKEAGKPCLFEEYGVTSDHCAVEKPWQNTALNTTAISGDLYWQYGDQLSGGPSPDDGNTFYYGTDDFKCLVTDHIAAINSRN |
Enzyme Length | 386 |
Uniprot Accession Number | B8NVK8 |
Absorption | |
Active Site | ACT_SITE 208; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:Q99036; ACT_SITE 316; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q99036 |
Activity Regulation | |
Binding Site | BINDING 95; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 207; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 283; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 346; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07 |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.; EC=3.2.1.78; |
DNA Binding | |
EC Number | 3.2.1.78 |
Enzyme Function | FUNCTION: Endo-1,4-mannanase, a crucial enzyme for depolymerization of seed galactomannans and wood galactoglucomannans. {ECO:0000250}. |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Active site (2); Binding site (4); Chain (1); Erroneous initiation (1); Glycosylation (1); Signal peptide (1) |
Keywords | Carbohydrate metabolism;Glycoprotein;Glycosidase;Hydrolase;Reference proteome;Secreted;Signal |
Interact With | |
Induction | |
Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | SIGNAL 1..21; /evidence=ECO:0000255 |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 42,008 |
Kinetics | |
Metal Binding | |
Rhea ID | |
Cross Reference Brenda |