| IED ID | IndEnz0008000135 |
| Enzyme Type ID | cellulase000135 |
| Protein Name |
Mannan endo-1,4-beta-mannosidase 4 EC 3.2.1.78 Beta-mannanase 4 Endo-beta-1,4-mannanase 4 LeMAN4a LeMAN4i |
| Gene Name | MAN4 |
| Organism | Solanum lycopersicum (Tomato) (Lycopersicon esculentum) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae asterids lamiids Solanales Solanaceae Solanoideae Solaneae Solanum Solanum subgen. Lycopersicon Solanum lycopersicum (Tomato) (Lycopersicon esculentum) |
| Enzyme Sequence | MNNSIILIFVAILIIFPNEFSKPTRAFSNNNFVYTDGTHFALNGKSLYINGFNAYWLMYIAYDPSTRIKVTNTFQQASKYKMNVARTWAFSHGGSRPLQSAPGVYNEQMFQGLDFVISEAKKYGIHLIMSLVNNWDAFGGKKQYVEWAVQRGQKLTSDDDFFTNPMVKGFYKNNVKVVLTRVNTITKVAYKDDPTILSWELINEPRCPSDLSGKTFQNWVLEMAGYLKSIDSNHLLEIGLEGFYGNDMRQYNPNSYIFGTNFISNNQVQGIDFTTIHMYPNQWLPGLTQEAQDKWASQWIQVHIDDSKMLKKPLLIAEFGKSTKTPGYTVAKRDNYFEKIYGTIFNCAKSGGPCGGGLFWQVLGQGMSSFDDGYQVVLQESPSTSRVILLQSLRLSKLS |
| Enzyme Length | 399 |
| Uniprot Accession Number | Q8L5J1 |
| Absorption | |
| Active Site | ACT_SITE 204; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:Q99036; ACT_SITE 318; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q99036 |
| Activity Regulation | |
| Binding Site | BINDING 88; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 203; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 279; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 360; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.; EC=3.2.1.78; |
| DNA Binding | |
| EC Number | 3.2.1.78 |
| Enzyme Function | FUNCTION: Possesses endo-beta-mannanase and mannan transglycosylase activities. May be involved in cell wall degradation during fruit ripening. {ECO:0000269|PubMed:12427992, ECO:0000269|PubMed:16649044}. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 5.0 for both endo-beta-mannanase and mannan transglycosylase activities. {ECO:0000269|PubMed:16649044}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Beta strand (14); Binding site (4); Chain (1); Disulfide bond (1); Helix (17); Mutagenesis (6); Natural variant (1); Sequence conflict (1); Signal peptide (1); Turn (2) |
| Keywords | 3D-structure;Direct protein sequencing;Disulfide bond;Glycosidase;Hydrolase;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..26; /evidence="ECO:0000269|PubMed:12427992, ECO:0000269|PubMed:16649044" |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 1RH9; |
| Mapped Pubmed ID | 19450903; |
| Motif | |
| Gene Encoded By | |
| Mass | 45,339 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.2.1.78; |