| IED ID | IndEnz0008000140 |
| Enzyme Type ID | cellulase000140 |
| Protein Name |
Probable glucan 1,3-beta-glucosidase D EC 3.2.1.58 Exo-1,3-beta-glucanase D |
| Gene Name | exgD NFIA_054930 |
| Organism | Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) (Aspergillus fischerianus) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Fumigati Aspergillus fischeri Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) (Aspergillus fischerianus) |
| Enzyme Sequence | MPSHSRSRDRYGGRDSDREARYDYDYARRRYATDDNDDDYDDDELEHGLNERRYRRDGYLPPRESRTRGYYERDAEGAADEELLGDERNPGPRASRSYGHDYDARRGERSRAREAPRRSERHRDRDREGQSRRRAYEDDGRHRTRDGRRERGRESDAEARRSRRREAGRETAARKHQSSDSTNSASHLLSADALARLGAQYEKEERRKRENAKDAAKAERKRQKKRAVVGEESRALRDPPGESHRDRTKARVASGAYLEEGRSPEMQVRHRGGGGPAMEARWRKEGSWGGTMDDAGGGRPFWKRKKWIGLGALILILVIVIPVAVVVSKKHDNKSDPADPQGTSPGKSNLDGLSHDSIPAYAQGTYLDPWTWYDTTDFNVTFTNETVGGLSIMGLNSTWDDSARPNDNVPPLNEPFPYGSQPIRGVNLGGWLSIEPFIVPSLFDSYSSVAGIIDEWTLSKRLGSSAARTLEKHYATFITEQDFADIRDAGLDHVRIQYSYWAVATYDDDPYVAKISWRYLLRAIEYCRKYGLRVKLDPHGIPGSQNGWNHSGREGVIGWLNGTDGELNRNRSLAVHDSVSKFFAQDRYKNIVTIYGLVNEPLMLSLSVEDVLDWTTEATKLVQKNGITAYVALHDGFLNLSKWKSMLKNRPDKMLLDTHQYTIFNTGQIGLNHTAKVNLICNDWYNMIKEINSTSTGWGPTICGEWSQADTDCAKYLNNVGRGTRWEGTFSLTDSTQYCPTADTGPPCSCANANADVSEYSADYKKFLQTYAEAQMSAFETGQGWFYWTWRTESAAQWSYRTAWKNGFMPAKAYAPSFKCGDAVPDFGNLPEYY |
| Enzyme Length | 834 |
| Uniprot Accession Number | A1DMX4 |
| Absorption | |
| Active Site | ACT_SITE 600; /note=Proton donor; /evidence=ECO:0000250; ACT_SITE 705; /note=Nucleophile; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1->3)-beta-D-glucans, releasing alpha-glucose.; EC=3.2.1.58; |
| DNA Binding | |
| EC Number | 3.2.1.58 |
| Enzyme Function | FUNCTION: Glucosidase involved in the degradation of cellulosic biomass. Active on lichenan (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (5); Glycosylation (10); Region (3); Topological domain (2); Transmembrane (1) |
| Keywords | Carbohydrate metabolism;Cell membrane;Cell wall biogenesis/degradation;Glycoprotein;Glycosidase;Hydrolase;Membrane;Polysaccharide degradation;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass type II membrane protein {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 94,751 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |