Detail Information for IndEnz0008000146
IED ID IndEnz0008000146
Enzyme Type ID cellulase000146
Protein Name Mannan endo-1,4-beta-mannosidase 5
EC 3.2.1.78
Beta-mannanase 5
Endo-beta-1,4-mannanase 5
LeMAN5
Gene Name MAN5
Organism Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae asterids lamiids Solanales Solanaceae Solanoideae Solaneae Solanum Solanum subgen. Lycopersicon Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Enzyme Sequence MAYFQRLISCIFVLFLLSLAFACEARVLLDENNANDQGFVRVNGAHFELNGSPFLFNGFNSYWLMHVAAEPSERYKVSEVLREASSAGLSVCRTWAFSDGGDRALQISPGVYDERVFQGLDFVISEAKKYGIRLILSFVNNYNDFGGKAQYVQWARNAGAQINGDDDFYTNYITKNYYKNHIKKVVTRFNTITGMTYKDDSTIMAWELMNEPRNQADYSGNTLNAWVQEMASFVKSLDNKHLLEIGMEGFYGDSVPERKSINPGYQVGTDFISNHLIKEIDFATIHAYTDQWLSGQSDDAQMIFMQKWMTSHWQDAKNILKKPLVLAEFGKSSRDPGYNQNIRDTFMSTIYRNIYSLAKDGGTMGGSLIWQLVAQGMENYEDGYCIELGKNPSTAGIITSQSHAMTALAHLVKI
Enzyme Length 414
Uniprot Accession Number Q6YM50
Absorption
Active Site ACT_SITE 211; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:Q99036; ACT_SITE 328; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q99036
Activity Regulation
Binding Site BINDING 95; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 210; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 288; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 370; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.; EC=3.2.1.78;
DNA Binding
EC Number 3.2.1.78
Enzyme Function FUNCTION: May be involved in weakening of anther wall during pollen development.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Binding site (4); Chain (1); Signal peptide (1)
Keywords Glycosidase;Hydrolase;Reference proteome;Secreted;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..25; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 46,835
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda 3.2.1.78;