| IED ID | IndEnz0008000149 |
| Enzyme Type ID | cellulase000149 |
| Protein Name |
Probable mannan endo-1,4-beta-mannosidase F EC 3.2.1.78 Endo-beta-1,4-mannanase F |
| Gene Name | manF AN9276 |
| Organism | Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Nidulantes Emericella nidulans (Aspergillus nidulans) Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) |
| Enzyme Sequence | MKRQALTLIPLLGAAAAQSGPYGQCGGNDWSGATTCVSGYVCVYQNEWYSQCVPGTATSSSTTLTTTTSATTRTTTTTTSTTSVPSSTNFPSASGLNFTIDGVTDYFAGSNSYWISMLTNDADVDLVLDHIASSGHKILRIWGFNDVNTEPSTGQVWFQKHQGGVSTINTGQYGLQRLDAVVSSAEKRGIKLIINFVNNWDDYGGMSAYLNAYGGSTKTDWYTSATIQAAYRTYIKAVIDRFIDSPAIFAWELANEPRCNGCDTSILYDWIADTSAYIKSLDPLHMVCIGDEGFGLDEGSDGSYPFSYNEGLDFAANLAIDTIDFGTFHLYPGSWGVSYDWGNLWAITHGAACATAGKPCLFEEYGAPSDHCAIEVPWQTTAVSSTGIAGDLFWQWGDTLSTGQTHNDGNTIYYGSDEYTCMVTEHMERIAAR |
| Enzyme Length | 433 |
| Uniprot Accession Number | Q5AR04 |
| Absorption | |
| Active Site | ACT_SITE 256; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:Q99036; ACT_SITE 364; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q99036 |
| Activity Regulation | |
| Binding Site | BINDING 142; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 255; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 331; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 394; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.; EC=3.2.1.78; |
| DNA Binding | |
| EC Number | 3.2.1.78 |
| Enzyme Function | FUNCTION: Endo-1,4-mannanase, a crucial enzyme for depolymerization of seed galactomannans and wood galactoglucomannans. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Binding site (4); Chain (1); Domain (1); Erroneous gene model prediction (2); Glycosylation (1); Region (2); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Glycoprotein;Glycosidase;Hydrolase;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 46,869 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |