| IED ID | IndEnz0008000150 |
| Enzyme Type ID | cellulase000150 |
| Protein Name |
Probable mannan endo-1,4-beta-mannosidase F EC 3.2.1.78 Endo-beta-1,4-mannanase F |
| Gene Name | manF NFIA_099770 |
| Organism | Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) (Aspergillus fischerianus) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Fumigati Aspergillus fischeri Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) (Aspergillus fischerianus) |
| Enzyme Sequence | MRPLSSAALLSAIGAVAAQVGPWGQCGGQSYTGGTSCVSGWACVFLNDWYSQCQPGAEYTPPLCGRNDNPNIFQATTTSTSVSATAPPSSTSSSTASVSSSTSSTPIPTSSGSFVKAEGLKFNIDGETKYFAGTNAYWLPFLTNNADVDSVFDHLQQTGLKILRTWGFNDVNSVPNPGTVYFQLHDPSTSTTTINTGADGLQRLDYVVSAAEKHGIKLLIPLVNNWDDYGGMNAYIKAYGGSKTEWYTNSKIQSVYQAYIKAVVSRYRDSPAIMAWELSNEARCQGCSTDVIYNWATKTSAYIKSLDPNHMVATGEEGMGLTVDSDGSYPYSTYEGSDFEKNLAIPHIDFGVFHLYTADWGITDNSWGNRWVTSHAKLCEAAGKPCLFEEYGLKDDHCSAAVVWQKTSLTTAGMAADLFWQYGQTLSTGQSPNDRYTIYYGTSDWQCAVIDHVSRI |
| Enzyme Length | 456 |
| Uniprot Accession Number | A1DBV1 |
| Absorption | |
| Active Site | ACT_SITE 281; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:Q99036; ACT_SITE 390; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q99036 |
| Activity Regulation | |
| Binding Site | BINDING 166; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 280; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 356; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 420; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.; EC=3.2.1.78; |
| DNA Binding | |
| EC Number | 3.2.1.78 |
| Enzyme Function | FUNCTION: Endo-1,4-mannanase, a crucial enzyme for depolymerization of seed galactomannans and wood galactoglucomannans. {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Binding site (4); Chain (1); Domain (1); Region (3); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Glycosidase;Hydrolase;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..18; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 49,510 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |