| IED ID | IndEnz0008000161 |
| Enzyme Type ID | cellulase000161 |
| Protein Name |
Serine/threonine-protein kinase OXI1 EC 2.7.11.1 AGC serine/threonine-protein kinase subfamily 2 member 1 Protein OXIDATIVE SIGNAL-INDUCIBLE 1 |
| Gene Name | OXI1 AGC2-1 At3g25250 MJL12.21 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MLEGDEKQSRALDFNRLEVLSLLGRGAKGVVFLVRDDDAKLLALKVILKEAIEKKKKGRESEDDEYKRVSFEQGVLSRFDHPLFPSLHGVLATDKVIGYAIDYCPGQNLNSLRKMQSESMFSDEIIRFYAAELVLALDYLHNQGIVYRDLKPDNVMIQENGHLMLIDFDLSTNLAPRTPQPSPSLSKPSPTMKRKKRLFRFTSFCNSGISPQESISVHSSSTLAVSDSSGEKSNSFVGTEEYVAPEVISGDGHDFAVDWWSLGVVLYEMLYGATPFRGSNRKETFYRILSKPPNLTGETTSLRDLIRRLLEKDPSRRINVEEIKGHDFFRGVDWEKVILVSRPPYIPAPDDGGDKGTDVNTKMDVENIVQEIFAARQEREKQSGDNNKNANMKIKDNTSGEWVKGLNNNHDLESDNNFLVF |
| Enzyme Length | 421 |
| Uniprot Accession Number | Q9LSF1 |
| Absorption | |
| Active Site | ACT_SITE 149; /note="Proton acceptor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU00159, ECO:0000255|PROSITE-ProRule:PRU10027" |
| Activity Regulation | ACTIVITY REGULATION: Activated in response to hydrogen peroxide and cellulase elicitor. Activated by PDK1 in a phosphatidic acid dependent manner. {ECO:0000269|PubMed:14749726, ECO:0000269|PubMed:14985766}. |
| Binding Site | BINDING 45; /note=ATP; /evidence=ECO:0000255|PROSITE-ProRule:PRU00159 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616, ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1; CATALYTIC ACTIVITY: Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060, Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013, ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216; EC=2.7.11.1; |
| DNA Binding | |
| EC Number | 2.7.11.1 |
| Enzyme Function | FUNCTION: Involved in oxidative burst-mediated signaling. Required for basal resistance to P.parasitica infection and root hair growth. Partly required for the activation of MPK3 and MPK6 by hydrogen peroxide and cellulase elicitor. {ECO:0000269|PubMed:14985766}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 23..31; /note=ATP; /evidence=ECO:0000255|PROSITE-ProRule:PRU00159 |
| Features | Active site (1); Binding site (1); Chain (1); Domain (2); Modified residue (1); Motif (1); Mutagenesis (4); Nucleotide binding (1); Region (1) |
| Keywords | ATP-binding;Kinase;Nucleotide-binding;Phosphoprotein;Plant defense;Reference proteome;Serine/threonine-protein kinase;Transferase |
| Interact With | O80719 |
| Induction | INDUCTION: By wounding, hydrogen peroxide and cellulase elicitor. {ECO:0000269|PubMed:14985766}. |
| Subcellular Location | |
| Modified Residue | MOD_RES 235; /note=Phosphoserine; by PDPK1; /evidence=ECO:0000305 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 12383089; 15235117; 15713015; 16146321; 16219920; 16258017; 16280546; 16289945; 16632591; 17040918; 17092320; 17227549; 17337630; 17665211; 18702669; 18775970; 18783601; 19574254; 19704505; 20561255; 20736450; 20739304; 21175636; 21276203; 21419340; 21625539; 23278806; 23343093; 23818851; 25763633; 26373654; 26594223; 26747288; 27212106; 27247031; 27398933; 27806116; 27862280; 29575603; 29777502; 31123095; 31519798; 32796124; |
| Motif | MOTIF 418..421; /note=PIF |
| Gene Encoded By | |
| Mass | 47,560 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:17989; RHEA:46608 |
| Cross Reference Brenda |