| IED ID | IndEnz0008000165 |
| Enzyme Type ID | cellulase000165 |
| Protein Name |
Galactose-1-phosphate uridylyltransferase Gal-1-P uridylyltransferase EC 2.7.7.12 UDP-glucose--hexose-1-phosphate uridylyltransferase |
| Gene Name | gal7 |
| Organism | Hypocrea jecorina (Trichoderma reesei) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Hypocreomycetidae Hypocreales Hypocreaceae Trichoderma Hypocrea jecorina (Trichoderma reesei) |
| Enzyme Sequence | MPDKILDDISHRRYNPLTDSWLLVSPHRTKRPWQGQQEGAAVTTLPEYDPKCYLCPGNSRAAGDQNPNYEQTFAFVNDYSAVKEQQPDYEVDQSSDDLESLLLRAQGVKGVCYVLTFSPKHNVTLADMSAKDILPTINHWTRLYANHLSPSNPLSAVAAQLQLPISKEEAPVPKDNYRYMQIFENKGAAMGCSNPHPHCQAWTTSTMPEEPGKELVQMAKYRQQHGRHLLADYIKLELAKEERVVWQNDSFVVVCPWWAIWPFEVLVLPKRHVRALVDLTADERLQLAEAIQEVTRRYDNLFECHFPYSSGIHQAPLDGTPEEIENAYFHMHFYPPLLRSATVKKFLVGFELMAEAQRDITPEQATIRLRACDGELYRNKLS |
| Enzyme Length | 382 |
| Uniprot Accession Number | Q96UI1 |
| Absorption | |
| Active Site | ACT_SITE 198; /note=Tele-UMP-histidine intermediate; /evidence=ECO:0000255|PROSITE-ProRule:PRU10033 |
| Activity Regulation | |
| Binding Site | BINDING 61; /note=UDP-alpha-D-glucose; via amide nitrogen; /evidence=ECO:0000250|UniProtKB:P07902; BINDING 185; /note=UDP-alpha-D-glucose; shared with dimeric partner; /evidence=ECO:0000250|UniProtKB:P07902; BINDING 200; /note=UDP-alpha-D-glucose; /evidence=ECO:0000250|UniProtKB:P07902 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=alpha-D-galactose 1-phosphate + UDP-alpha-D-glucose = alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose; Xref=Rhea:RHEA:13989, ChEBI:CHEBI:58336, ChEBI:CHEBI:58601, ChEBI:CHEBI:58885, ChEBI:CHEBI:66914; EC=2.7.7.12; Evidence={ECO:0000250|UniProtKB:P09148}; |
| DNA Binding | |
| EC Number | 2.7.7.12 |
| Enzyme Function | FUNCTION: Essential for growth on galactose but not for cellulase induction. {ECO:0000269|PubMed:11919723}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Carbohydrate metabolism; galactose metabolism. |
| nucleotide Binding | |
| Features | Active site (1); Binding site (3); Chain (1); Metal binding (8); Region (3) |
| Keywords | Carbohydrate metabolism;Galactose metabolism;Iron;Metal-binding;Nucleotidyltransferase;Transferase;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 43,781 |
| Kinetics | |
| Metal Binding | METAL 52; /note=Zinc; /evidence=ECO:0000255|PROSITE-ProRule:PRU10033; METAL 55; /note=Zinc; /evidence=ECO:0000255|PROSITE-ProRule:PRU10033; METAL 121; /note=Zinc; /evidence=ECO:0000255|PROSITE-ProRule:PRU10033; METAL 196; /note=Zinc; /evidence=ECO:0000255|PROSITE-ProRule:PRU10033; METAL 214; /note=Iron; /evidence=ECO:0000250|UniProtKB:P09148; METAL 313; /note=Iron; /evidence=ECO:0000250|UniProtKB:P09148; METAL 330; /note=Iron; /evidence=ECO:0000250|UniProtKB:P09148; METAL 332; /note=Iron; /evidence=ECO:0000250|UniProtKB:P09148 |
| Rhea ID | RHEA:13989 |
| Cross Reference Brenda |