| IED ID | IndEnz0008000169 |
| Enzyme Type ID | cellulase000169 |
| Protein Name |
Ergosteryl-beta-glucosidase EC 3.2.1.- |
| Gene Name | EGH1 YIR007W YIB7W |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Enzyme Sequence | MPAKIHISADGQFCDKDGNEIQLRGVNLDPSVKIPAKPFLSTHAPIENDTFFEDADKVSFINHPLVLDDIEQHIIRLKSLGYNTIRLPFTWESLEHAGPGQYDFDYMDYIVEVLTRINSVQQGMYIYLDPHQDVWSRFSGGSGAPLWTLYCAGFQPANFLATDAAILHNYYIDPKTGREVGKDEESYPKMVWPTNYFKLACQTMFTLFFGGKQYAPKCTINGENIQDYLQGRFNDAIMTLCARIKEKAPELFESNCIIGLESMNEPNCGYIGETNLDVIPKERNLKLGKTPTAFQSFMLGEGIECTIDQYKRTFFGFSKGKPCTINPKGKKAWLSAEERDAIDAKYNWERNPEWKPDTCIWKLHGVWEIQNGKRPVLLKPNYFSQPDATVFINNHFVDYYTGIYNKFREFDQELFIIIQPPVMKPPPNLQNSKILDNRTICACHFYDGMTLMYKTWNKRIGIDTYGLVNKKYSNPAFAVVLGENNIRKCIRKQLSEMQKDAKSMLGKKVPVFFTEIGIPFDMDDKKAYITNDYSSQTAALDALGFALEGSNLSYTLWCYCSINSHIWGDNWNNEDFSIWSPDDKPLYHDTRAKTPTPEPSPASTVASVSTSTSKSGSSQPPSFIKPDNHLDLDSPSCTLKSDLSGFRALDAIMRPFPIQIHGRFEFAEFNLCNKSYLLKLVGKTTPEQITVPTYIFIPRHHFTPSRLSIRSSSGHYTYNTDYQVLEWFHEPGHQFIEICAKSKSRPNTPGSDTSNDLPAECVIS |
| Enzyme Length | 764 |
| Uniprot Accession Number | P40566 |
| Absorption | |
| Active Site | ACT_SITE 515; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q99036 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=ergosteryl 3-beta-D-glucoside + H2O = D-glucose + ergosterol; Xref=Rhea:RHEA:24640, ChEBI:CHEBI:4167, ChEBI:CHEBI:15377, ChEBI:CHEBI:16933, ChEBI:CHEBI:52973; Evidence={ECO:0000269|PubMed:26116408}; |
| DNA Binding | |
| EC Number | 3.2.1.- |
| Enzyme Function | FUNCTION: Ergosteryl beta-glucosidase involved in the ergosteryl beta-glucoside (EG) catabolic pathway and vacuole formation via hydrolysis of EG to generate glucose (PubMed:26116408). Is also able to hydrolyze cholesteryl beta-glucoside and sitosteryl beta-glucoside to generate glucose; and C6-7-nitro-2,1,3-benzoxadiazole (NBD)-GlcCer to generate C6-NBD-ceramide (Cer) (PubMed:26116408). {ECO:0000269|PubMed:26116408}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Compositional bias (1); Modified residue (1); Region (1) |
| Keywords | Cytoplasm;Glycosidase;Hydrolase;Membrane;Phosphoprotein;Reference proteome;Vacuole |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000269|PubMed:26116408}. Vacuole membrane {ECO:0000269|PubMed:26116408}; Peripheral membrane protein {ECO:0000269|PubMed:26116408}. Note=Localizes in the cytosol until the initial logarithmic phase, then targets to a yet unidentified organelle with a granule structure in the middle logarithmic phase, and finally localizes at the vacuole membranes in the stationary phase. {ECO:0000269|PubMed:26116408}. |
| Modified Residue | MOD_RES 594; /note=Phosphothreonine; /evidence=ECO:0007744|PubMed:19779198 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 12140549; 14562095; 21216897; 21918511; |
| Motif | |
| Gene Encoded By | |
| Mass | 86,979 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:24640 |
| Cross Reference Brenda | 3.2.1.104; |