Detail Information for IndEnz0008000170
IED ID IndEnz0008000170
Enzyme Type ID cellulase000170
Protein Name Probable endo-beta-1,4-glucanase D
Endoglucanase D
EC 3.2.1.4
Carboxymethylcellulase D
Cellulase D
Gene Name eglD AO090005000531
Organism Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Circumdati Aspergillus oryzae (Yellow koji mold) Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Enzyme Sequence MKSSTFGMLALAAAAKLVSAHATVHAVWINDVDQGEGNSESGYIRSPPSNSPITDVTSKDMTCNVNNKATAKTLEVKAGDKITFEWHHDSRSDSDDIIASSHKGPIMVYMAPTEKGTAGNGWVKIAEDGYTDGTWAVDTLIKNRGKHSVTVPDVAAGEYLFRPEIIALHEGNRQGGAQFYMECVQVKVTSSGSKTLPEGVSIPGAYTATDKGILFDIYNSFDSYPFPGPAVWDGASGSSSSPSASASASAPAATSAAPAPSSFTTIAKQPATSSTEAPSTENTSTTSTIVSTTAAASATAPATPSSTSAIASSAASTNSVPQPSSNAGGAVKEWYQCGGLNYKGSTQCEEGLTCKKWNPYYYQCISA
Enzyme Length 367
Uniprot Accession Number Q2US83
Absorption
Active Site ACT_SITE 164; /note=Proton donor; /evidence=ECO:0000250; ACT_SITE 210; /note=Nucleophile; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
DNA Binding
EC Number 3.2.1.4
Enzyme Function FUNCTION: Has endoglucanase activity on substrates containing beta-1,4 glycosidic bonds, like in carboxymethylcellulose (CMC), hydroxyethylcellulose (HEC) and beta-glucan. Involved in the degradation of complex natural cellulosic substrates (By similarity). {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Disulfide bond (2); Domain (1); Glycosylation (1); Region (4); Signal peptide (1)
Keywords Carbohydrate metabolism;Cellulose degradation;Disulfide bond;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Secreted;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..20; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 38,033
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda