| IED ID | IndEnz0008000173 |
| Enzyme Type ID | cellulase000173 |
| Protein Name |
Mannan endo-1,4-beta-mannosidase A EC 3.2.1.78 Beta-mannanase 5A Man5A Beta-mannanase I/II BMANI BMANII Endo-beta-1,4-mannanase A |
| Gene Name | man1 |
| Organism | Hypocrea jecorina (strain ATCC 56765 / BCRC 32924 / NRRL 11460 / Rut C-30) (Trichoderma reesei) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Hypocreomycetidae Hypocreales Hypocreaceae Trichoderma Hypocrea jecorina (Trichoderma reesei) Hypocrea jecorina (strain ATCC 56765 / BCRC 32924 / NRRL 11460 / Rut C-30) (Trichoderma reesei) |
| Enzyme Sequence | MMMLSKSLLSAATAASALAAVLQPVPRASSFVTISGTQFNIDGKVGYFAGTNCYWCSFLTNHADVDSTFSHISSSGLKVVRVWGFNDVNTQPSPGQIWFQKLSATGSTINTGADGLQTLDYVVQSAEQHNLKLIIPFVNNWSDYGGINAYVNAFGGNATTWYTNTAAQTQYRKYVQAVVSRYANSTAIFAWELGNEPRCNGCSTDVIVQWATSVSQYVKSLDSNHLVTLGDEGLGLSTGDGAYPYTYGEGTDFAKNVQIKSLDFGTFHLYPDSWGTNYTWGNGWIQTHAAACLAAGKPCVFEEYGAQQNPCTNEAPWQTTSLTTRGMGGDMFWQWGDTFANGAQSNSDPYTVWYNSSNWQCLVKNHVDAINGGTTTPPPVSSTTTTSSRTSSTPPPPGGSCSPLYGQCGGSGYTGPTCCAQGTCIYSNYWYSQCLNT |
| Enzyme Length | 437 |
| Uniprot Accession Number | Q99036 |
| Absorption | |
| Active Site | ACT_SITE 196; /note=Proton donor/acceptor; /evidence=ECO:0000305|PubMed:10666621; ACT_SITE 303; /note=Nucleophile; /evidence=ECO:0000305|PubMed:10666621 |
| Activity Regulation | |
| Binding Site | BINDING 232; /note="Substrate"; /evidence="ECO:0000269|PubMed:10666621, ECO:0007744|PDB:1QNR"; BINDING 274; /note="Substrate"; /evidence="ECO:0000269|PubMed:10666621, ECO:0007744|PDB:1QNR" |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.; EC=3.2.1.78; Evidence={ECO:0000269|PubMed:8529653, ECO:0000269|Ref.3, ECO:0000269|Ref.4}; |
| DNA Binding | |
| EC Number | 3.2.1.78 |
| Enzyme Function | FUNCTION: Endo-1,4-mannanase that catalyzes the random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans and heteromannans. It is a crucial enzyme for depolymerization of seed galactomannans and wood galactoglucomannans. Active against locust bean gum and ivory nut mannan, releasing mainly tri- and disaccharides (PubMed:7793911, Ref.3, Ref.4, PubMed:8529653). Also has transglycosylation activity. Transglycosylation of two mannotrioses into a mannohexaose is the major transglycosylation route (PubMed:8529653, Ref.7, PubMed:24950755). {ECO:0000269|PubMed:24950755, ECO:0000269|PubMed:7793911, ECO:0000269|PubMed:8529653, ECO:0000269|Ref.3, ECO:0000269|Ref.4, ECO:0000269|Ref.7}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 75 degrees Celsius. {ECO:0000269|Ref.3}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 5.0. Stable from pH 2.5 to 7.0. {ECO:0000269|Ref.3}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Beta strand (15); Binding site (2); Chain (1); Compositional bias (1); Disulfide bond (4); Domain (1); Glycosylation (4); Helix (14); Mutagenesis (1); Propeptide (1); Region (4); Signal peptide (1); Site (1); Turn (1) |
| Keywords | 3D-structure;Direct protein sequencing;Disulfide bond;Glycoprotein;Glycosidase;Hydrolase;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|Ref.3, ECO:0000269|Ref.4}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (5) |
| Cross Reference PDB | 1QNO; 1QNP; 1QNQ; 1QNR; 1QNS; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 47,053 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=0.0015 mg/ml for locust bean gum mannan {ECO:0000269|Ref.3}; KM=0.6 mg/ml for locust bean gum mannan {ECO:0000269|Ref.7}; KM=0.25 mM for 4-methylumbelliferyl-mannotrioside {ECO:0000269|Ref.7}; KM=0.31 mM for mannotetraose {ECO:0000269|Ref.7}; KM=0.08 mM for mannopentaose {ECO:0000269|Ref.7}; KM=0.05 mM for mannohexaose {ECO:0000269|Ref.7}; |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.2.1.78; |