| IED ID | IndEnz0008000200 |
| Enzyme Type ID | cellulase000200 |
| Protein Name |
Glucosylceramidase EC 3.2.1.45 Endoglycoceramidase-related protein 1 EGCrP1 |
| Gene Name | egc1 CDV57_02266 |
| Organism | Neosartorya fumigata (Aspergillus fumigatus) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Fumigati Neosartorya fumigata (Aspergillus fumigatus) |
| Enzyme Sequence | MGVLRLRVDGQTFRDPDNREITLRGINVAGEAKYPKKPDIPSYVSDGFFDADNVSFVGRPFSLDDAHTHFSRLRKWGYNTIRYVFTWEAIEHEGPGKYDDEWISFTIEVLRIAKQYGFYVFLDPHQDVWSRLSGGSGAPAWTLYAAGFDPRGFKKTEAALVQNTFDDPAEFPKMIWSTNYTRLVSQTMFTLFYGGRDFAPKAIIDGINIQDYLQGHFIAACRYFAQKIHEAGDIENEVVIGWESMNEPNRGLIGVQDISVIPPEQQLQLGTSPTAFQGMLTGSGRACEESTWAFGGFGPYQTGRELVDPEGETAWLPADYDDTRYGWVRDPGWKLGECLWAQHGVWDPSSDKLLRKDYFAKNPQTGEPLNYDKFTNTYFMEHYRAYRDALRSVWPEAIIFCQPPVMEVPPDLKGTVDDDPNMVHAVHYYDGITLLTKHWNRLYNVDVIGVLRGKYLTPAFAVKIGETAIRNCLRDQLKFLRDESLRYMGTHPLIFTEIGIPFDMDDRHAYKTGDYSGQVSAMDANHFAIEGSTANGFTLWLYTTSNNHEWGDNWNGEDLSIYSVDDLELPSGKLLAFENESQRDPQSPAYSESQRNTESYRVGPRDLKQALQAPSISSEISQSSQDKLGFRAAEAWVRPSPIITNGQILQYGFDLKSCVFSMRLLGEKKGLGQEAATEIFLPDFHFPDTHTVVAVSAGEWTIDYPEIHSVKFQRLRWWHPEGDHNIKIQGVKRKPGDPTVSGEELSYLEQCQGGGCSVM |
| Enzyme Length | 759 |
| Uniprot Accession Number | H1AE13 |
| Absorption | |
| Active Site | ACT_SITE 247; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:O85465; ACT_SITE 497; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:O85465 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose; Xref=Rhea:RHEA:13269, ChEBI:CHEBI:4167, ChEBI:CHEBI:15377, ChEBI:CHEBI:22801, ChEBI:CHEBI:52639; EC=3.2.1.45; Evidence={ECO:0000269|PubMed:22072709};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:13270; Evidence={ECO:0000269|PubMed:22072709}; |
| DNA Binding | |
| EC Number | 3.2.1.45 |
| Enzyme Function | FUNCTION: Specifically hydrolyzes the glucosidic linkage in glucosylceramide (PubMed:22072709). May prevent accumulation of aberrent glucosylceramide containing immature ceramide (By similarity). {ECO:0000250|UniProtKB:H1AE12, ECO:0000269|PubMed:22072709}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (1); Region (1) |
| Keywords | Glycosidase;Hydrolase;Membrane |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Peripheral membrane protein {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 86,064 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:13269; RHEA:13270 |
| Cross Reference Brenda |