| IED ID | IndEnz0008000219 |
| Enzyme Type ID | cellulase000219 |
| Protein Name |
Endoglucanase 4 EC 3.2.1.4 Cellulase 4 Endo-1,4-beta-glucanase 4 Endoglucanase IV EG-IV |
| Gene Name | Eg IV |
| Organism | Ruminococcus albus |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Clostridia Eubacteriales Oscillospiraceae Ruminococcus Ruminococcus albus |
| Enzyme Sequence | MLDKLKVINGKLTAGEKPVRLFGLSTHGIAWYPEYICEESFNALKKDWRTNCIRIAMYTDEFRGYCKDGNKQHLKELIEKGVVIAEKLDMYVIVDWHVLCDQDPMKYIDEAEEFFSDMSKRFANKTNVIYEICNEPNCSGTWDKITEYADRIIPIIRSNSPDALIVTGTSTWSQDIHCALEKPLKWDNVMYSLHFYAATHKGTLRSRLERCIEAGLPVFINEFNLCEASGKGDIDIDEANAWYEVIDRLGLSCISWCLSNSGDTCGVFTQNCTKLSGWTDEDIKTSGKIIKGWFEAFADEENTNEQCFRIDK |
| Enzyme Length | 312 |
| Uniprot Accession Number | Q07940 |
| Absorption | |
| Active Site | ACT_SITE 135; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:O85465; ACT_SITE 222; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:O85465 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; |
| DNA Binding | |
| EC Number | 3.2.1.4 |
| Enzyme Function | |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 40 degrees Celsius.; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7.; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1) |
| Keywords | Carbohydrate metabolism;Cellulose degradation;Direct protein sequencing;Glycosidase;Hydrolase;Polysaccharide degradation |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 35,767 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |