| IED ID | IndEnz0008000228 |
| Enzyme Type ID | cellulase000228 |
| Protein Name |
Probable endo-beta-1,4-glucanase celB Endoglucanase celB EC 3.2.1.4 Carboxymethylcellulase celB Cellulase B |
| Gene Name | celB ACLA_066030 |
| Organism | Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Fumigati Aspergillus clavatus Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) |
| Enzyme Sequence | MVRTFAVTALALLPLVAAQQIGSTKEVHPQLTTYKCTSQGGCVKQNTSIVLDSGSHWIHAKGGEVSCTTSSGLDPALCPDKETCAENCVVEGITDYSQYGVQTRGDAMLLREYIKQNNQTKAPSPRVYLLDEDGENYSMLRLLNQEFTFDVDVSKLPCGMNGALYFSEMSASGGRSALNPAGAAYGTGYCDAQCYTNAWINGEANTAKAGLCCQEMDIWEANARANAFTPHPCNSTGLLGCAGDECNSVCDKAGCGFNPYALGARDYYGTAMTVDTTKPFTVVTQFLTADNSTTGALREIRRLYVQAGQVIQNAVVKVDGRTVNSITEPYCASQGVFEGLGGLRRMGEALGRGMVLSMSIWNDAGGFMHWLDSGNSGPCSSTEGDPSLIENKYPDTAVTFSKIRWGDLGTTFATRRLH |
| Enzyme Length | 418 |
| Uniprot Accession Number | A1CG87 |
| Absorption | |
| Active Site | ACT_SITE 215; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 220; /note=Proton donor; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; |
| DNA Binding | |
| EC Number | 3.2.1.4 |
| Enzyme Function | FUNCTION: Has endoglucanase activity on substrates containing beta-1,4 glycosidic bonds, like in carboxymethylcellulose (CMC), hydroxyethylcellulose (HEC) and beta-glucan. Involved in the degradation of complex natural cellulosic substrates (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Glycosylation (5); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Cellulose degradation;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..18; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 44,827 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |