| IED ID | IndEnz0008000233 |
| Enzyme Type ID | cellulase000233 |
| Protein Name |
Mannan endo-1,4-beta-mannosidase F EC 3.2.1.78 Endo-beta-1,4-mannanase F |
| Gene Name | manF AFUA_8G07030 |
| Organism | Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Fumigati Neosartorya fumigata (Aspergillus fumigatus) Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus) |
| Enzyme Sequence | MHPLPSVALLSAIGAVAAQVGPWGQCGGRSYTGETSCVSGWSCVLFNEWYSQCQPATTTSTSSVSATAAPSSTSSSKESVPSATTSKKPVPTGSSSFVKADGLKFNIDGETKYFAGTNAYWLPFLTNDADVDSVMDNLQKAGLKILRTWGFNDVNSKPSSGTVYFQLHDPSTGTTTINTGADGLQRLDYVVSAAEKRGIKLLIPLVNNWDDYGGMNAYVKAYGGSKTEWYTNSKIQSVYQAYIKAVVSRYRDSPAIMAWELSNEARCQGCSTDVIYNWTAKTSAYIKSLDPNHMVATGDEGMGVTVDSDGSYPYSTYEGSDFAKNLAAPDIDFGVFHLYTEDWGIKDNSWGNGWVTSHAKVCKAAGKPCLFEEYGLKDDHCSASLTWQKTSVSSGMAADLFWQYGQTLSTGPSPNDHFTIYYGTSDWQCGVADHLSTL |
| Enzyme Length | 438 |
| Uniprot Accession Number | Q4WBS1 |
| Absorption | |
| Active Site | ACT_SITE 264; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:Q99036; ACT_SITE 373; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q99036 |
| Activity Regulation | |
| Binding Site | BINDING 149; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 263; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 339; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 402; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.; EC=3.2.1.78; |
| DNA Binding | |
| EC Number | 3.2.1.78 |
| Enzyme Function | FUNCTION: Endo-1,4-mannanase, a crucial enzyme for depolymerization of seed galactomannans and wood galactoglucomannans. {ECO:0000269|PubMed:15541293, ECO:0000269|PubMed:19205049}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 65 degrees Celsius. Stable up to 55 degrees Celsius. {ECO:0000269|PubMed:15541293, ECO:0000269|PubMed:19205049}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 4.0 to 5.0. {ECO:0000269|PubMed:15541293, ECO:0000269|PubMed:19205049}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Binding site (4); Chain (1); Domain (1); Erroneous initiation (1); Glycosylation (1); Region (3); Sequence conflict (6); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Direct protein sequencing;Glycoprotein;Glycosidase;Hydrolase;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..17; /evidence=ECO:0000269|PubMed:15541293 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 47,327 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=3.07 mM for locust bean galactomannan {ECO:0000269|PubMed:15541293, ECO:0000269|PubMed:19205049}; Vmax=1935 umol/min/mg enzyme {ECO:0000269|PubMed:15541293, ECO:0000269|PubMed:19205049}; |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.2.1.78; |