| IED ID | IndEnz0008000234 |
| Enzyme Type ID | cellulase000234 |
| Protein Name |
Probable mannan endo-1,4-beta-mannosidase F EC 3.2.1.78 Endo-beta-1,4-mannanase F |
| Gene Name | manF AO090038000444 |
| Organism | Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Circumdati Aspergillus oryzae (Yellow koji mold) Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) |
| Enzyme Sequence | MRSLSSIALLSVVGAASAQAGPWAQCGGKSFSGSSECASGWKCQELNEWFSQCVPGAESTTPTVSSTPTPTDAPSVSITASVTTGINKSISVSSASKSTPLPSSSSASPSPRPTGSGSFAKADGLQFSIDGETKYFAGTNAYWLPFQMNDADIDSVFDHLEQAGLKILRVWGFNDVNTAPSPGTVYFQLHDKEKGTSTINTGKDGLQRLDYVVAAAEKHGVKLIIPFVNSWDDYGGYNAYVKAYGGSKTEWFTNEKIQSVYQAYIKAVVSRYRDSPAIFAWELGNEPRCSGCSTDVIHGWATKISAYIKSLDPNHMVALGDEGMGLTIGSDQSYPYGTSEGNDFEKNLAIPDIDFGTLHLYTTDWGIKDNAWGNGWVENHAKACKAAGKPCLFEEYGMKGNHCTDELKWQKTSLSSGTAADLIWQYGQQLSTGESPKDAYSIFYGTDEWKCAVMDHMENVNKN |
| Enzyme Length | 463 |
| Uniprot Accession Number | Q2U2I3 |
| Absorption | |
| Active Site | ACT_SITE 286; /note=Proton donor/acceptor; /evidence=ECO:0000250|UniProtKB:Q99036; ACT_SITE 395; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q99036 |
| Activity Regulation | |
| Binding Site | BINDING 171; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 285; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 361; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 424; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.; EC=3.2.1.78; |
| DNA Binding | |
| EC Number | 3.2.1.78 |
| Enzyme Function | FUNCTION: Endo-1,4-mannanase, a crucial enzyme for depolymerization of seed galactomannans and wood galactoglucomannans. {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Binding site (4); Chain (1); Compositional bias (1); Domain (1); Erroneous gene model prediction (1); Glycosylation (1); Region (4); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Glycoprotein;Glycosidase;Hydrolase;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..18; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 49,992 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |