Detail Information for IndEnz0008000235
IED ID IndEnz0008000235
Enzyme Type ID cellulase000235
Protein Name Mannan endo-1,4-beta-mannosidase 1
EC 3.2.1.78
Beta-mannanase 1
Endo-beta-1,4-mannanase 1
OsMAN1
Gene Name MAN1 Os01g0663300 LOC_Os01g47400 P0671D01.40
Organism Oryza sativa subsp. japonica (Rice)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Oryzoideae Oryzeae Oryzinae Oryza Oryza sativa (Rice) Oryza sativa subsp. japonica (Rice)
Enzyme Sequence MRLLGAHRAALLVLACVVVVVIHGLGEAEALGGGGGFVRAQGTRFVLDGNPYYANGFNAYWLMLLAADPSQRGKVSAALGEAAGHGLTVARTWAFSDGGGGNALQLSPGNYNENTFKGLDFVLSEARKYGIKVILSLVDNYDSFGGRKQYVNWARAQGQGIGSDDEFFTNPVVKGFYKNHVKTVLTRKNTITGVAYRDDPTILAWELMNEPRCQSDLSGRTVQSWITEMAAHVKSIDRNHMLEVGLEGFYGASSPSRIAAVNPSGYQLGTDFIANNQVPGIDFATVHSYPDQWLSGKDDQAQLGFMGRWLDAHIADAQAVLRKPLLIAEFGKSWKDPGYSSGQRDALYGTVYAKIYESARRGGATVGGLFWQLLVPGMDSYRDGYEVVFGETPSTTGVITTNSRRLRFLSKAFARARQAQPARGKGRHNGGK
Enzyme Length 432
Uniprot Accession Number Q0JKM9
Absorption
Active Site ACT_SITE 210; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:Q99036; ACT_SITE 329; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q99036
Activity Regulation
Binding Site BINDING 93; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 209; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 289; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 371; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.; EC=3.2.1.78;
DNA Binding
EC Number 3.2.1.78
Enzyme Function
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Alternative sequence (1); Binding site (4); Chain (1); Signal peptide (1)
Keywords Alternative splicing;Glycosidase;Hydrolase;Reference proteome;Secreted;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..28; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 46,875
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda