| IED ID | IndEnz0008000243 |
| Enzyme Type ID | cellulase000243 |
| Protein Name |
Mannan endo-1,4-beta-mannosidase C EC 3.2.1.78 Endo-beta-1,4-mannanase C |
| Gene Name | manC AN6427 |
| Organism | Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Nidulantes Emericella nidulans (Aspergillus nidulans) Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) |
| Enzyme Sequence | MIFSTLLSLALLATTATARKGFVTTKGDKFQLDGKDFYFAGSNAYYFPFNNNQTDVELGLSAAKKAGLLVFRTWGFNDKNVTYIEDGLPQYGGEGAGTTEVVFQWWQNGTSTIDLEPFDKVVNAAAKTGIKLIVTLVNNWADYGGMDVYTVNLGGQYHDDFYRLPQIKKAYKRYVKEMVTRYRNSPAIMAWELANEPRCGADGVRNLPASDECTPELLTSWIDEMSTYVKRLDPHHLVTWGGEGGFNYDSDDWAYNGSDGGDFEAELKLKNIDFGVFHSYPDWWSKTVEWTNKWIVDHARAARRVGKPVVHEEYGWLTPQGRLDNLGTVSNITRLEAVGGWQSISLREKMSDMFWQFGYSGYSYGRNHDDGFTIYLDDAEAQELVYKHAKEVNKLNRRR |
| Enzyme Length | 399 |
| Uniprot Accession Number | Q5AZ53 |
| Absorption | |
| Active Site | ACT_SITE 196; /note=Proton donor/acceptor; /evidence=ECO:0000250|UniProtKB:Q99036; ACT_SITE 313; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q99036 |
| Activity Regulation | |
| Binding Site | BINDING 74; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 195; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 243; /note=Substrate; /evidence=ECO:0000250|UniProtKB:Q99036; BINDING 280; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 284; /note=Substrate; /evidence=ECO:0000250|UniProtKB:Q99036; BINDING 355; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.; EC=3.2.1.78; Evidence={ECO:0000269|PubMed:16844780}; |
| DNA Binding | |
| EC Number | 3.2.1.78 |
| Enzyme Function | FUNCTION: Endo-1,4-mannanase that catalyzes the random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans and heteromannans. It is a crucial enzyme for depolymerization of seed galactomannans and wood galactoglucomannans. Active against locust bean gum and gum guar (PubMed:16844780). Also has transglycosylation activity. Produces mainly mannopentaose and mannohexaose out of mannotetraose (PubMed:21867780, PubMed:24950755). {ECO:0000269|PubMed:16844780, ECO:0000269|PubMed:21867780, ECO:0000269|PubMed:24950755}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 50 degrees Celsius. {ECO:0000269|PubMed:21867780}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 5.5. {ECO:0000269|PubMed:21867780}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Binding site (6); Chain (1); Disulfide bond (1); Glycosylation (5); Region (1); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Disulfide bond;Glycoprotein;Glycosidase;Hydrolase;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q99036}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..18; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 45,338 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=3.1 mg/ml for locust bean gum mannan {ECO:0000269|PubMed:24950755}; KM=1.8 mM for mannopentaose {ECO:0000269|PubMed:21867780}; KM=0.6 mM for mannohexaose {ECO:0000269|PubMed:21867780}; |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.2.1.78; |