Detail Information for IndEnz0008000243
IED ID IndEnz0008000243
Enzyme Type ID cellulase000243
Protein Name Mannan endo-1,4-beta-mannosidase C
EC 3.2.1.78
Endo-beta-1,4-mannanase C
Gene Name manC AN6427
Organism Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Nidulantes Emericella nidulans (Aspergillus nidulans) Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Enzyme Sequence MIFSTLLSLALLATTATARKGFVTTKGDKFQLDGKDFYFAGSNAYYFPFNNNQTDVELGLSAAKKAGLLVFRTWGFNDKNVTYIEDGLPQYGGEGAGTTEVVFQWWQNGTSTIDLEPFDKVVNAAAKTGIKLIVTLVNNWADYGGMDVYTVNLGGQYHDDFYRLPQIKKAYKRYVKEMVTRYRNSPAIMAWELANEPRCGADGVRNLPASDECTPELLTSWIDEMSTYVKRLDPHHLVTWGGEGGFNYDSDDWAYNGSDGGDFEAELKLKNIDFGVFHSYPDWWSKTVEWTNKWIVDHARAARRVGKPVVHEEYGWLTPQGRLDNLGTVSNITRLEAVGGWQSISLREKMSDMFWQFGYSGYSYGRNHDDGFTIYLDDAEAQELVYKHAKEVNKLNRRR
Enzyme Length 399
Uniprot Accession Number Q5AZ53
Absorption
Active Site ACT_SITE 196; /note=Proton donor/acceptor; /evidence=ECO:0000250|UniProtKB:Q99036; ACT_SITE 313; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q99036
Activity Regulation
Binding Site BINDING 74; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 195; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 243; /note=Substrate; /evidence=ECO:0000250|UniProtKB:Q99036; BINDING 280; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 284; /note=Substrate; /evidence=ECO:0000250|UniProtKB:Q99036; BINDING 355; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.; EC=3.2.1.78; Evidence={ECO:0000269|PubMed:16844780};
DNA Binding
EC Number 3.2.1.78
Enzyme Function FUNCTION: Endo-1,4-mannanase that catalyzes the random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans and heteromannans. It is a crucial enzyme for depolymerization of seed galactomannans and wood galactoglucomannans. Active against locust bean gum and gum guar (PubMed:16844780). Also has transglycosylation activity. Produces mainly mannopentaose and mannohexaose out of mannotetraose (PubMed:21867780, PubMed:24950755). {ECO:0000269|PubMed:16844780, ECO:0000269|PubMed:21867780, ECO:0000269|PubMed:24950755}.
Temperature Dependency BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 50 degrees Celsius. {ECO:0000269|PubMed:21867780};
PH Dependency BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 5.5. {ECO:0000269|PubMed:21867780};
Pathway
nucleotide Binding
Features Active site (2); Binding site (6); Chain (1); Disulfide bond (1); Glycosylation (5); Region (1); Signal peptide (1)
Keywords Carbohydrate metabolism;Disulfide bond;Glycoprotein;Glycosidase;Hydrolase;Reference proteome;Secreted;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q99036}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..18; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 45,338
Kinetics BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=3.1 mg/ml for locust bean gum mannan {ECO:0000269|PubMed:24950755}; KM=1.8 mM for mannopentaose {ECO:0000269|PubMed:21867780}; KM=0.6 mM for mannohexaose {ECO:0000269|PubMed:21867780};
Metal Binding
Rhea ID
Cross Reference Brenda 3.2.1.78;