Detail Information for IndEnz0008000253
IED ID IndEnz0008000253
Enzyme Type ID cellulase000253
Protein Name Mannan endo-1,4-beta-mannosidase 8
EC 3.2.1.78
Beta-mannanase 8
Endo-beta-1,4-mannanase 8
OsMAN8
Gene Name MAN8 Os12g0117300 LOC_Os12g02520
Organism Oryza sativa subsp. japonica (Rice)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Oryzoideae Oryzeae Oryzinae Oryza Oryza sativa (Rice) Oryza sativa subsp. japonica (Rice)
Enzyme Sequence MVECSGTQLWASGRPFIIHGFNTYWLMSFAADQATRPRVTAAIAEAAEAGLNVCRTWAFSDGGYRALQTVPFHYDEDVFQALDFVVSEAKRHNMRLILSLCNNWEDYGGKAQYVRWGKEAGLDLTSEDDFFSDPTIKSYYKAFVEAVVTRINTVTNETYKDDPTILAWELINEPRCPSDPSGDTLQAWIEEMASYVKSIDPVHLLEIGIEGFYGLSTPELLPVNPDEYSGHAGTDFIRNHQAPGIDLASIHVYSDTWLPHSIKENHLQFVDKWMQQHIHDAANLLGMPIVVGEFGVSVKDGKFGNEFREDFMKTVYRIFLSSWKEGVIGGGCLLWQLFPEGAEHMDDGYAVIFAKSPSTLSLLANHLRCLEC
Enzyme Length 372
Uniprot Accession Number Q0IQJ7
Absorption
Active Site ACT_SITE 173; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:Q99036; ACT_SITE 293; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q99036
Activity Regulation
Binding Site BINDING 57; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 172; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 253; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 335; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.; EC=3.2.1.78;
DNA Binding
EC Number 3.2.1.78
Enzyme Function
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Binding site (4); Chain (1); Erroneous gene model prediction (1)
Keywords Glycosidase;Hydrolase;Reference proteome
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 41,839
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda