| IED ID | IndEnz0008000253 |
| Enzyme Type ID | cellulase000253 |
| Protein Name |
Mannan endo-1,4-beta-mannosidase 8 EC 3.2.1.78 Beta-mannanase 8 Endo-beta-1,4-mannanase 8 OsMAN8 |
| Gene Name | MAN8 Os12g0117300 LOC_Os12g02520 |
| Organism | Oryza sativa subsp. japonica (Rice) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Oryzoideae Oryzeae Oryzinae Oryza Oryza sativa (Rice) Oryza sativa subsp. japonica (Rice) |
| Enzyme Sequence | MVECSGTQLWASGRPFIIHGFNTYWLMSFAADQATRPRVTAAIAEAAEAGLNVCRTWAFSDGGYRALQTVPFHYDEDVFQALDFVVSEAKRHNMRLILSLCNNWEDYGGKAQYVRWGKEAGLDLTSEDDFFSDPTIKSYYKAFVEAVVTRINTVTNETYKDDPTILAWELINEPRCPSDPSGDTLQAWIEEMASYVKSIDPVHLLEIGIEGFYGLSTPELLPVNPDEYSGHAGTDFIRNHQAPGIDLASIHVYSDTWLPHSIKENHLQFVDKWMQQHIHDAANLLGMPIVVGEFGVSVKDGKFGNEFREDFMKTVYRIFLSSWKEGVIGGGCLLWQLFPEGAEHMDDGYAVIFAKSPSTLSLLANHLRCLEC |
| Enzyme Length | 372 |
| Uniprot Accession Number | Q0IQJ7 |
| Absorption | |
| Active Site | ACT_SITE 173; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:Q99036; ACT_SITE 293; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q99036 |
| Activity Regulation | |
| Binding Site | BINDING 57; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 172; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 253; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07; BINDING 335; /note=Substrate; /evidence=ECO:0000250|UniProtKB:B4XC07 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.; EC=3.2.1.78; |
| DNA Binding | |
| EC Number | 3.2.1.78 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Binding site (4); Chain (1); Erroneous gene model prediction (1) |
| Keywords | Glycosidase;Hydrolase;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 41,839 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |