| IED ID | IndEnz0008000262 |
| Enzyme Type ID | cellulase000262 |
| Protein Name |
Endoglucanase 2 EC 3.2.1.4 Endo-1,4-beta glucanase 2 OsCel9A OsGLU5 |
| Gene Name | GLU5 Os01g0220100 LOC_Os01g12070 OsJ_000889 P0483F08.13 P0489G09.28 |
| Organism | Oryza sativa subsp. japonica (Rice) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Oryzoideae Oryzeae Oryzinae Oryza Oryza sativa (Rice) Oryza sativa subsp. japonica (Rice) |
| Enzyme Sequence | MARGGGAAGVSMAHHLGIALVVLVFAAMAQVARGGGGGHDYGMALSKSILYFEAQRSGVLPGSQRIAWRANSGLADGKANGVDLVGGYYDAGDNVKFGLPMAFTVTMMAWSVIEYGEEMAAAGELGHAVEAIKWGTDYFAKAHPEPNVLYAEVGDGDSDHNCWQRPEDMTTSRQAYRLDPQNPGSDLAGETAAAMAAASLVFRSSNPGYADQLLQHSKQLFDFADKYRGRYDNSITVARNYYGSFSGYGDELLWASAWLYQASDDRRYLDYLANNADALGGTGWSINQFGWDVKYPGVQILAAKFLLQGKAGEHAGVLQGYRRKADFFACSCLGKDAADNVGRTPGGMLYHQRWNNIQFVTSASFLLAVYSDHLAGGAVRCSGGGGAVAGAAELLAFAKSQVDYILGSNPRGTSYMVGYGAVYPRQAHHRGSSIASIRASPSFVSCREGYASWYGRRGGNPNLLDGAVVGGPDEHDDFADERNNYEQTEAATYNNAPLMGILARLAAGHGARARGRLGQSLQHGIAANHTSLPHGANHQHASPVEIEQKATASWEKDGRTYHRYAVTVSNRSPAGGKTVEELHIGIGKLYGPVWGLEKAARYGYVLPSWTPSLPAGESAAFVYVHAAPPADVWVTGYKLV |
| Enzyme Length | 640 |
| Uniprot Accession Number | Q5NAT0 |
| Absorption | |
| Active Site | ACT_SITE 93; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10140; ACT_SITE 428; /evidence=ECO:0000255|PROSITE-ProRule:PRU10059; ACT_SITE 480; /evidence=ECO:0000255|PROSITE-ProRule:PRU10060; ACT_SITE 489; /evidence=ECO:0000255|PROSITE-ProRule:PRU10060 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; |
| DNA Binding | |
| EC Number | 3.2.1.4 |
| Enzyme Function | FUNCTION: Hydrolyzes 1,4-beta-glycosyl linkages of 1,4-beta-glucans and 1,3-1,4-beta-glucans. Possesses broad substrate specificity for hemicelluloses of type II cell walls. Substrate preference is carboxymethyl-cellulose > 1,3-1,4-beta-glucan > lichenan > arabinoxylan > phospho-swollen cellulose > xylan > glucomannan. May participate in lateral root development. {ECO:0000269|PubMed:17056618, ECO:0000269|PubMed:17056619}. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 5.3-5.6 with 1,3-1,4-beta-glucan as substrate (at 35 degrees Celsius). {ECO:0000269|PubMed:17056618}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (4); Chain (1); Glycosylation (1); Propeptide (1); Sequence conflict (1); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Cell wall biogenesis/degradation;Cellulose degradation;Direct protein sequencing;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | INDUCTION: By auxin in roots (at protein level). {ECO:0000269|PubMed:17056618}. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..34; /evidence=ECO:0000269|PubMed:17056618 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 68,408 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |