IED ID | IndEnz0008000276 |
Enzyme Type ID | cellulase000276 |
Protein Name |
Endoglucanase A EC 3.2.1.4 Endo-1,4-beta-glucanase A |
Gene Name | eglA |
Organism | Bacillus pumilus (Bacillus mesentericus) |
Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus pumilus (Bacillus mesentericus) |
Enzyme Sequence | MLIFETYLILFKTVQITKRRIERRRLRLLNQCFTKKEGVSNREMASYNYVEVLQKSMLFYEAQRSGRLPESNRLNWRGDSGLKDGKDVGHDLTGGWYDAGDHVKFGLPMAYSAAVLAWTVYEYREAYEEAELLDEILDQIKWATDYFLKAHTGPNEFWAQVGDGNADHAWWGPAEVMPMNRPAFKIDEHCPGTEVAAQTAAALAAGSIIFKETDASYAAKLLTHAKQLYAFADRYRGKYTDCVTNAQPFYNSWSGYVDELIWGGIWLYLATNEETYLNKALKAVEEWPQDWDYTFTMSWDNTFFASQILLARITKENRFIESTERNLDYWTTGLVQNGKVERITYTPGGLAWLDQWGSLRYAANAAFLAFVYADWVSDQEKKNRYQSFAIKQTHYMLGDNPLNRSYVVGFGQNSPKHPHHRTAHGSWSNQLTNPPSHRHTLYGALVGGPNAQDQYDDDISDYISNEVATDYNAAFTGNIAKMVQLFGEGQSKLPNFPPKEQVEDEFFVEAAVMHNDTTSTQVKAVLYNRSGWPARSSQTLSFRYYVNLSEVFAKGFTEKDIQVTAAYNEGASLSPLKVYDASSRVYFAEIDFTGVVISPRGESEHKKEIQFRLSAPNGSNIWDASNDYSYQGLTSNMQKTTKIPVFEDGVLVFGTLPDK |
Enzyme Length | 659 |
Uniprot Accession Number | Q5YLG1 |
Absorption | |
Active Site | ACT_SITE 101; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10140; ACT_SITE 419; /evidence=ECO:0000255|PROSITE-ProRule:PRU10059; ACT_SITE 457; /evidence=ECO:0000255|PROSITE-ProRule:PRU10060; ACT_SITE 466; /evidence=ECO:0000255|PROSITE-ProRule:PRU10060 |
Activity Regulation | ACTIVITY REGULATION: Strongly inhibited by ZnCl(2) and by EDTA. {ECO:0000269|PubMed:15538558}. |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; Evidence={ECO:0000269|PubMed:15538558}; |
DNA Binding | |
EC Number | 3.2.1.4 |
Enzyme Function | FUNCTION: Active on carboxymethyl cellulose and carboxymethyl cellulose-RBB but not avicel, xanthan gum, carboxymethyl-curdulan-RBB or carboxymethyl-xylan-RBB. {ECO:0000269|PubMed:15538558}. |
Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 60 degrees Celsius. Retains more than 90% activity after 24 hours at 50 degrees Celsius. {ECO:0000269|PubMed:15538558}; |
PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 5-8. {ECO:0000269|PubMed:15538558}; |
Pathway | |
nucleotide Binding | |
Features | Active site (4); Chain (1); Domain (1); Region (2) |
Keywords | Carbohydrate metabolism;Cellulose degradation;Glycosidase;Hydrolase;Polysaccharide degradation;Secreted |
Interact With | |
Induction | |
Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:15538558}. |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 74,985 |
Kinetics | |
Metal Binding | |
Rhea ID | |
Cross Reference Brenda |