| IED ID | IndEnz0008000292 |
| Enzyme Type ID | cellulase000292 |
| Protein Name |
Endoglucanase/exoglucanase B Includes: Endoglucanase EC 3.2.1.4 Cellobiohydrolase Cellulase Endo-1,4-beta-glucanase ; Exoglucanase EC 3.2.1.91 1,4-beta-cellobiohydrolase Exocellobiohydrolase |
| Gene Name | celB |
| Organism | Caldicellulosiruptor saccharolyticus (Caldocellum saccharolyticum) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Clostridia Thermoanaerobacterales Thermoanaerobacterales Family III. Incertae Sedis Caldicellulosiruptor Caldicellulosiruptor saccharolyticus (Caldocellum saccharolyticum) |
| Enzyme Sequence | MKRNLFRIVSRVVLIAFIASISLVGAMSYFPVETQAAPDWSIPSLCESYKDDFMIGVAIPARCLSNDTDKRMVLKHFNSITAENEMKPESLLAGQTSTGLSYRFSTADAFVDFASTNKIGIRGHTLVWHNQTPDWFFKDSNGQRLSKDALLARLKQYIYDVVGRYKGKVYAWDVVNEAIDENQPDSYRRSTWYEICGPEYIEKAFIWAHEADPNAKLFYNDYNTEISKKRDFIYNMVKNLKSKGIPIHGIGMQCHINVNWPSVSEIENSIKLFSSIPGIEIHITELDMSLYNYGSSENYSTPPQDLLQKQSQKYKEIFTMLKKYKNVVKSVTFWGLKDDYSWLRSFYGKNDWPLLFFEDYSAKPAYWAVIEASGVTTSSPTPTPTPTVTVTPTPTPTPTPTVTATPTPTPTPVSTPATGGQIKVLYANKETNSTTNTIRPWLKVVNSGSSSIDLSRVTIRYWYTVDGERAQSAVSDWAQIGASNVTFKFVKLSSSVSGADYYLEIGFKSGAGQLQPGKDTGEIQIRFNKSDWSNYNQGNDWSWLQSMTSYGENEKVTAYIDGVLVWGQEPSGATPAPTMTVAPTATPTPTLSPTVTPTPAPTQTAIPTPTLTPNPTPTSSIPDDTNDDWLYVSGNKIVDKDGRPVWLTGINWFGYNTGTNVFDGVWSCNLKDTLAEIANRGFNLLRVPISAELILNWSQGIYPKPNINYYVNPELEGKNSLEVFDIVVQTCKEVGLKIMLDIHSIKTDAMGHIYPVWYDEKFTPEDFYKACEWITNRYKNDDTIIAFDLKNEPHGKPWQDTTFAKWDNSTDINNWKYAAETCAKRILNINPNLLIVIEGIEAYPKDDVTWTSKSSSDYYSTWWGGNLRGVRKYPINLGKYQNKVVYSPHDYGPSVYQQPWFYPGFTKESLLQDCWRPNWAYIMEENIAPLLIGEWGGHLDGADNEKWMKYLRDYIIENHIHHTFWCFNANSGDTGGLVGYDFTTWDEKKYSFLKPALWQDSQGRFVGLDHKRPLGTNGKNINITTYYNNNEPEPVPASK |
| Enzyme Length | 1039 |
| Uniprot Accession Number | P10474 |
| Absorption | |
| Active Site | ACT_SITE 177; /note=Proton donor; /evidence=ECO:0000255; ACT_SITE 285; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10061; ACT_SITE 792; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.; EC=3.2.1.91; |
| DNA Binding | |
| EC Number | 3.2.1.4; 3.2.1.91 |
| Enzyme Function | FUNCTION: This protein is made up of two domains: the N-terminal domain has exoglucanase activity while the C-terminal domain is an endoglucanase. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Compositional bias (2); Domain (2); Region (2); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Cellulose degradation;Glycosidase;Hydrolase;Multifunctional enzyme;Polysaccharide degradation;Repeat;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..28 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 117,641 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.2.1.4;3.2.1.91; |