| IED ID | IndEnz0008000295 |
| Enzyme Type ID | cellulase000295 |
| Protein Name |
Endoglucanase B EC 3.2.1.4 Cellulase B Endo-1,4-beta-glucanase B Endo-1,4-beta-xylanase EC 3.2.1.8 |
| Gene Name | engB Clocel_1150 |
| Organism | Clostridium cellulovorans (strain ATCC 35296 / DSM 3052 / OCM 3 / 743B) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Clostridia Eubacteriales Clostridiaceae Clostridium Clostridium cellulovorans Clostridium cellulovorans (strain ATCC 35296 / DSM 3052 / OCM 3 / 743B) |
| Enzyme Sequence | MNKRLSRGKISLLASVFVTTTFMGGVNVLASTAKTGIRDITSQQVVKEMKVGWNLGNTMDATGGETNWGNPLTTHAMIDKVKAAGFNTLRLPITWDGHIGAAPDYAIDATWMNRVEEIANYAFDNNMYVIINLHHEDGWLKPYYANEAEVKAKITKVWTQIANRFKDYGDYLIFETMNEPRPVGAADEWSGGSYENRDMVNRYNLTAVNTIRATGGNNALRHIMVPTLAAAALSTTMNDYIVPNNDSRVIVSLHMYSPYFFSADLTSQWTTATWGSDADKAALSADFDAVYNKFVKNGRAVVIGEMGTINKNNLDSRVKHAEYYAKEATVRGITPIWWDNGYCVAGKEQTFGIFNRKNLTWCCPEVMQAFIRGAGATQTQTSYSLGDVNKDGKVNAIDYAVLKSILLGTNTNVDLSVSDMNKDGKVNALDLAVLKKMLLS |
| Enzyme Length | 440 |
| Uniprot Accession Number | P28621 |
| Absorption | |
| Active Site | ACT_SITE 179; /note=Proton donor; /evidence=ECO:0000250; ACT_SITE 305; /note=Nucleophile; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.; EC=3.2.1.8; |
| DNA Binding | |
| EC Number | 3.2.1.4; 3.2.1.8 |
| Enzyme Function | FUNCTION: Has endoglucanase activity on carboxymethyl-cellulose (CMC), xylan and lichenan, but not Avicel. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Domain (1); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Cellulose degradation;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..33; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 17693494; |
| Motif | |
| Gene Encoded By | |
| Mass | 48,637 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |