| IED ID | IndEnz0008000304 |
| Enzyme Type ID | cellulase000304 |
| Protein Name |
Endoglucanase C EC 3.2.1.4 Cellulase C Endo-1,4-beta-glucanase C |
| Gene Name | cenC Celf_1537 |
| Organism | Cellulomonas fimi (strain ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Micrococcales Cellulomonadaceae Cellulomonas Cellulomonas fimi Cellulomonas fimi (strain ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547) |
| Enzyme Sequence | MVSRRSSQARGALTAVVATLALALAGSGTALAASPIGEGTFDDGPEGWVAYGTDGPLDTSTGALCVAVPAGSAQYGVGVVLNGVAIEEGTTYTLRYTATASTDVTVRALVGQNGAPYGTVLDTSPALTSEPRQVTETFTASATYPATPAADDPEGQIAFQLGGFSADAWTFCLDDVALDSEVELLPHTSFAESLGPWSLYGTSEPVFADGRMCVDLPGGQGNPWDAGLVYNGVPVGEGESYVLSFTASATPDMPVRVLVGEGGGAYRTAFEQGSAPLTGEPATREYAFTSNLTFPPDGDAPGQVAFHLGKAGAYEFCISQVSLTTSATPPPGYEPDTGPRVRVNQVGYLPFGPKRATLVTDAAEPVAWELRDADGVVVADGTSEPRGVEPSAAQAVHVLDFSDVTTQGAGYTLVADGETSRPFDIDGDLYQQLRYDALNYFYLARSGTEIEADVVGEEYAREAGHVGVAPNQGDTDVPCIGPRDYYDGWTCDYRLDVSGGWYDAGDHGKYVVNGGIAVGQLLQTYERALHAGTADALADGTLDVPEHGNDVPDVLDEARWELEWMLSMIVPEGEYAGMVHHKVHDEGWTGLPLLPADDPQARSLHRPSTAATLNLSAVAAQGARLLEPYDPQLAQTLLEAARTTWAAAQEHPALYAPGEAGADGGGAYNDSQVADEFYWAAAELYLTTGEDAFATAVTTSPLHTADVFTADGFGWGSVAALGRLDLATVPNELPGLDAVQSSVVEGAQEYLAAQAGQGFGSLYSPPGGEYVWGSSSQVANNLVVVATAYDLTGDERFRAATLEGLDYLFGRNALNQSYVTGWGEVASHQQHSRWFAHQLDPSLPSPPPGSLAGGPNSQAATWDPTTKAAFPDGCAPSACYVDEIQAWSTNELTVNWNSALSWVASWVADQGSAEPVPTAPVVTRQPVDATVALGADATFTAEASGVPAPTVRWQVRAGRGWKDVAGATGTTLTVRATARTDGTRYRAVFTNAAGSVESAVVRLTVERAAPVVTQHPADVRARVGTRAVFRAAADGYPTPCVVWQVRWGGGSWRPIPWATSTTLSVPVTVLAAGTEYRAVFTNAVGTAATEPAELAVQRPRS |
| Enzyme Length | 1101 |
| Uniprot Accession Number | P14090 |
| Absorption | |
| Active Site | ACT_SITE 506; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10140; ACT_SITE 831; /evidence=ECO:0000255|PROSITE-ProRule:PRU10059; ACT_SITE 882; /evidence=ECO:0000255|PROSITE-ProRule:PRU10060; ACT_SITE 891; /evidence=ECO:0000255|PROSITE-ProRule:PRU10060 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; |
| DNA Binding | |
| EC Number | 3.2.1.4 |
| Enzyme Function | FUNCTION: The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that cut the disaccharide cellobiose from the non-reducing end of the cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (4); Beta strand (22); Chain (1); Domain (4); Region (2); Signal peptide (1); Turn (1) |
| Keywords | 3D-structure;Carbohydrate metabolism;Cellulose degradation;Direct protein sequencing;Glycosidase;Hydrolase;Immunoglobulin domain;Polysaccharide degradation;Reference proteome;Repeat;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..32; /evidence=ECO:0000269|PubMed:1956299 |
| Structure 3D | NMR spectroscopy (3); X-ray crystallography (1) |
| Cross Reference PDB | 1CX1; 1GU3; 1ULO; 1ULP; |
| Mapped Pubmed ID | 10704194; 12079353; |
| Motif | |
| Gene Encoded By | |
| Mass | 115,216 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |