| IED ID | IndEnz0008000330 |
| Enzyme Type ID | cellulase000330 |
| Protein Name |
Endoglucanase EC 3.2.1.4 Alkaline cellulase Endo-1,4-beta-glucanase |
| Gene Name | |
| Organism | Bacillus sp. (strain KSM-635) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Bacillaceae Bacillus unclassified Bacillus (in: Bacteria) Bacillus sp. (strain KSM-635) |
| Enzyme Sequence | MKIKQIKQSLSLLLIITLIMSLFVPMASANTNESKSNAFPFSDVKKTSWSFPYIKDLYEQEVITGTSATTFSPTDSVTRAQFTVMLTRGLGLEASSKDYPFKDRKNWAYKEIQAAYEAGIVTGKTNGEFAPNENITREQMAAMAVRAYEYLENELSLPEEQREYNDSSSISTFAQDAVQKAYVLELMEGNTDGYFQPKRNSTREQSAKVISTLLWKVASHDYLYHTEAVKSPSEAGALQLVELNGQLTLAGEDGTPVQLRGMSTHGLQWFGEIVNENAFVALSNDWGSNMIRLAMYIGENGYATNPEVKDLVYEGIELAFEHDMYVIVDWHVHAPGDPRADVYSGAYDFFEEIADHYKDHPKNHYIIWELANEPSPNNNGGPGLTNDEKGWEAVKEYAEPIVEMLREKGDNMILVGNPNWSQRPDLSADNPIDAENIMYSVHFYTGSHGASHIGYPEGTPSSERSNVMANVRYALDNGVAVFATEWGTSQANGDGGPYFDEADVWLNFLNKHNISWANWSLTNKNEISGAFTPFELGRTDATDLDPGANQVWAPEELSLSGEYVRARIKGIEYTPIDRTKFTKLVWDFNDGTTQGFQVNGDSPNKESITLSNNNDALQIEGLNVSNDISEGNYWDNVRLSADGWSENVDILGATELTIDVIVEEPTTVSIAAIPQGPAAGWANPTRAIKVTEDDFESFGDGYKALVTITSEDSPSLETIATSPEDNTMSNIILFVGTEDADVISLDNITVSGTEIEIEVIHDEKGTATLPSTFEDGTRQGWDWHTESGVKTALTIEEANGSNALSWEYAYPEVKPSDGWATAPRLDFWKDELVRGTSDYISFDFYIDAVRASEGAISINAVFQPPANGYWQEVPTTFEIDLTELDSATVTSDELYHYEVKINIRDIEAITDDTELRNLLLIFADEDSDFAGRVFVDNVRFE |
| Enzyme Length | 941 |
| Uniprot Accession Number | P19424 |
| Absorption | |
| Active Site | ACT_SITE 373; /note=Proton donor; ACT_SITE 485; /note=Nucleophile |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; |
| DNA Binding | |
| EC Number | 3.2.1.4 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Beta strand (13); Chain (1); Domain (3); Helix (16); Signal peptide (1); Turn (4) |
| Keywords | 3D-structure;Carbohydrate metabolism;Cellulose degradation;Direct protein sequencing;Glycosidase;Hydrolase;Polysaccharide degradation;Repeat;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..29 |
| Structure 3D | X-ray crystallography (2) |
| Cross Reference PDB | 1G01; 1G0C; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 104,628 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |