| IED ID | IndEnz0008000331 |
| Enzyme Type ID | cellulase000331 |
| Protein Name |
Endoglucanase EC 3.2.1.4 Cellulase Endo-1,4-beta-glucanase Endo-K |
| Gene Name | |
| Organism | Bacillus sp. (strain KSM-330) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Bacillaceae Bacillus unclassified Bacillus (in: Bacteria) Bacillus sp. (strain KSM-330) |
| Enzyme Sequence | MVEKRKIFTVLCACGIGFTSYTSCISAAAIDNDTLINNGHKINSSIITNSSQVSAVAKEMKPFPQQVNYSGILKPNHVSQESLNNAVKNYYNDWKKKYLKNDLSSLPGGYYVKGEITGNPDGFRPLGTSEGQGYGMIITVLMAGHDSNAQTIYDGLFKTARAFKSSINPNLMGWVVADDKKAQGHFDSATDGDLDIAYSLLLAHKQWGSSGKINYLKEAQNMITKGIKASNVTKNNGLNLGDWGDKSTFDTRPSDWMMSHLRAFYEFTGDKTWLNVIDNLYNTYTNFTNKYSPKTGLISDFVVKNPPQPAPKDFLDESKYTDSYYYNASRVPLRIVMDYAMYGEKRGKVISDKVATWIKSKTKGNPSKIVDGYKLDGTNIGDYPTAVYVSPFIAAGTTNSKNQEWVNSGWDWMKNKKESYFSDSYNLLTMLFLTGNWWKPIPDEKKIQSPINLEVQSELKEQD |
| Enzyme Length | 463 |
| Uniprot Accession Number | P29019 |
| Absorption | |
| Active Site | ACT_SITE 130; /note=Proton donor; /evidence=ECO:0000250; ACT_SITE 191; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10058 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; |
| DNA Binding | |
| EC Number | 3.2.1.4 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 5.2. Active from pH 4.5 to 6.5.; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Propeptide (1); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Cellulose degradation;Direct protein sequencing;Glycosidase;Hydrolase;Polysaccharide degradation;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | PTM: The N- and the C-terminus may be subjected to proteolysis. |
| Signal Peptide | SIGNAL 1..27; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 51,883 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |