Detail Information for IndEnz0008000341
IED ID IndEnz0008000341
Enzyme Type ID cellulase000341
Protein Name Endoglucanase
EC 3.2.1.4
Cellulase
Endo-1,4-beta-glucanase
Gene Name egl
Organism Ralstonia solanacearum (Pseudomonas solanacearum)
Taxonomic Lineage cellular organisms Bacteria Proteobacteria Betaproteobacteria Burkholderiales Burkholderiaceae Ralstonia Ralstonia solanacearum (Pseudomonas solanacearum)
Enzyme Sequence MRRCMPLVAASVAALMLAGCGGGDGDPSLSTASVSATDTTTLKPAATSTTSSVWLTLAKDSAAFTVSGTRTVRYGAGSAWVEKSVSGSGRCTSTFFGKDPAAGVAKVCQLLQGTGTLLWRGVSLAGAEFGEGSLPGTYGSNYIYPSADSVTYYKNKGMNLVRLPFRWERLQPTLNQVFDANELSRLTGFVNAVTATGQTVLLDPHNYARYYGNVIGSSAVPNSAYADFWRRLATQFKSNPRVILGLMNEPNSMPTEQWLSGANAELAAIRSANASNVVFVPGNAWTGAHSWNQNWYGTPNGTVMKGINDPGHNLVFEVHQYLDGDSSGQSANCVSATIGAQRLQDFTTWLRSNGYRGFLGEFGAASNDTCNQAVSNMLTFVKNNADVWTGWAWWAGGPWWGGYMYSIEPSNGVDKPQMSVLAPYLK
Enzyme Length 426
Uniprot Accession Number P17974
Absorption
Active Site ACT_SITE 249; /note=Proton donor; /evidence=ECO:0000250; ACT_SITE 361; /note=Nucleophile; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
DNA Binding
EC Number 3.2.1.4
Enzyme Function
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Lipidation (2); Propeptide (1); Signal peptide (1)
Keywords Carbohydrate metabolism;Cell membrane;Cellulose degradation;Direct protein sequencing;Glycosidase;Hydrolase;Lipoprotein;Membrane;Palmitate;Polysaccharide degradation;Signal;Zymogen
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor {ECO:0000305}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..19
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 45,578
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda