| IED ID | IndEnz0008000342 |
| Enzyme Type ID | cellulase000342 |
| Protein Name |
Endoglucanase EC 3.2.1.4 Cellulase Endo-1,4-beta-glucanase |
| Gene Name | egl RSp0162 RS05516 |
| Organism | Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Betaproteobacteria Burkholderiales Burkholderiaceae Ralstonia Ralstonia solanacearum (Pseudomonas solanacearum) Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum) |
| Enzyme Sequence | MHRCMPLVAASMAALMLAGCGGGDGDTTLSTAAATDTTTLKTAATTSISPLWLTIAKDSAAFTVSGTRTVRYGAGSAWVAKSMSGTGQCTAAFFGKDPAAGVAKVCQVAQGTGTLLWRGVSLAGAEFGEGSLPGTYGSNYIYPSADSATYYKNKGMNLVRLPFRWERLQPTLNQALDANELSRLTGFVNAVTAAGQTVLLDPHNYARYYGNVIGSSAVPNSAYADFWRRVATQFKGNARVIFGLMNEPNSMPTEQWLSGANAALAAIRSANASNVVFVPGNAWTGAHSWNQNWYGTPNGTVMKGINDPGRNLVFEVHQYLDGDSSGQSASCVSATIGAERLQDFTNWLRSNGYRGFLGEFGAASNDTCNQAVANMLTFVKNNADVWTGWAWWAGGPWWGGYMYSIEPSNGVDKPQMSVLAPYLK |
| Enzyme Length | 424 |
| Uniprot Accession Number | P58599 |
| Absorption | |
| Active Site | ACT_SITE 247; /note=Proton donor; /evidence=ECO:0000250; ACT_SITE 359; /note=Nucleophile; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; |
| DNA Binding | |
| EC Number | 3.2.1.4 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Erroneous initiation (1); Lipidation (2); Propeptide (1); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Cell membrane;Cellulose degradation;Glycosidase;Hydrolase;Lipoprotein;Membrane;Palmitate;Plasmid;Polysaccharide degradation;Reference proteome;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000255|PROSITE-ProRule:PRU00303 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | Plasmid megaplasmid Rsp |
| Mass | 45,081 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |