Detail Information for IndEnz0008000351
IED ID IndEnz0008000351
Enzyme Type ID cellulase000351
Protein Name Exoglucanase 1
EC 3.2.1.91
1,4-beta-cellobiohydrolase I
Beta-glucancellobiohydrolase I
Exocellobiohydrolase I
Exoglucanase I
Gene Name CBH-1
Organism Cryphonectria parasitica (Chestnut blight fungus) (Endothia parasitica)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Sordariomycetidae Diaporthales Cryphonectriaceae Cryphonectria-Endothia species complex Cryphonectria Cryphonectria parasitica (Chestnut blight fungus) (Endothia parasitica)
Enzyme Sequence MFSKFALTGSLLAGAVNAQGVGTQQTETHPQMTWQSCTSPSSCTTNQGEVVIDSNWRWVHDKDGYVNCYTGNTWNTTLCPDDKTCAANCVLDGADYSSTYGITTSGNALSLQFVTQSSGKNIGSRTYLMESSTKYHLFDLIGNEFAFDVDLSKLPCGLNGALYFVTMDADGGMAKYSTNTAGAEYGTGYCDSQCPRDLKFINGQGNVEGWTPSTNDANAGVGGLGSCCSEMDVWEANSMDMAYTPHPCETAAQHSCNADECGGTYSSSRYAGDCDPDGCDWNPFRMGNKDFYGSGDTVDTSQKFTVVTQFHGSGSSLTEISQYYIQGGTKIQQPNSTWPTLTGYNSITDDFCKAQKVEFNDTDVFSEKGGLAQMGAGMADGMVLVMSLWDDHYANMLWLDSTYPVDADASSPGKQRGTCATTSGVPADVESSDASATVIYSNIKFGPIGATY
Enzyme Length 452
Uniprot Accession Number Q00548
Absorption
Active Site ACT_SITE 230; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 235; /note=Proton donor; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.; EC=3.2.1.91;
DNA Binding
EC Number 3.2.1.91
Enzyme Function FUNCTION: The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that cut the disaccharide cellobiose from the non-reducing end of the cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Glycosylation (3); Signal peptide (1)
Keywords Carbohydrate metabolism;Cellulose degradation;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Signal
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..18; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 48,358
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda