| IED ID | IndEnz0008000361 |
| Enzyme Type ID | cellulase000361 |
| Protein Name |
Exoglucanase 2 EC 3.2.1.91 1,4-beta-cellobiohydrolase Cellobiohydrolase 6A Cel6A Exocellobiohydrolase II CBHII Exoglucanase II |
| Gene Name | cbh2 |
| Organism | Hypocrea jecorina (Trichoderma reesei) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Hypocreomycetidae Hypocreales Hypocreaceae Trichoderma Hypocrea jecorina (Trichoderma reesei) |
| Enzyme Sequence | MIVGILTTLATLATLAASVPLEERQACSSVWGQCGGQNWSGPTCCASGSTCVYSNDYYSQCLPGAASSSSSTRAASTTSRVSPTTSRSSSATPPPGSTTTRVPPVGSGTATYSGNPFVGVTPWANAYYASEVSSLAIPSLTGAMATAAAAVAKVPSFMWLDTLDKTPLMEQTLADIRTANKNGGNYAGQFVVYDLPDRDCAALASNGEYSIADGGVAKYKNYIDTIRQIVVEYSDIRTLLVIEPDSLANLVTNLGTPKCANAQSAYLECINYAVTQLNLPNVAMYLDAGHAGWLGWPANQDPAAQLFANVYKNASSPRALRGLATNVANYNGWNITSPPSYTQGNAVYNEKLYIHAIGPLLANHGWSNAFFITDQGRSGKQPTGQQQWGDWCNVIGTGFGIRPSANTGDSLLDSFVWVKPGGECDGTSDSSAPRFDSHCALPDALQPAPQAGAWFQAYFVQLLTNANPSFL |
| Enzyme Length | 471 |
| Uniprot Accession Number | P07987 |
| Absorption | |
| Active Site | ACT_SITE 245; /note=Proton donor; /evidence=ECO:0000269|PubMed:12188666 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.; EC=3.2.1.91; Evidence={ECO:0000269|Ref.3}; |
| DNA Binding | |
| EC Number | 3.2.1.91 |
| Enzyme Function | FUNCTION: Exocellobiohydrolases (CBH) that catalyzes the hydrolysis of 1,4-beta-D-glucosidic bonds in cellulose to release the disaccharide cellobiose (Ref.3). The degradation of cellulose involves an interplay between different cellulolytic enzymes. Hydrolysis starts with endoglucanases (EGs), which cut internal beta-1,4-glucosidic bonds in cellulose to reduce the polymerization degree of the substrate and create new chain ends for exocellobiohydrolases (CBHs). The CBHs release the disaccharide cellobiose from the non-reducing end of the cellulose polymer chain. Finally, beta-1,4-glucosidases hydrolyze the cellobiose and other short cello-oligosaccharides into glucose units (Probable). {ECO:0000269|Ref.3, ECO:0000305}. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 4-6. {ECO:0000269|PubMed:12188666}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (14); Chain (1); Disulfide bond (2); Domain (1); Glycosylation (9); Helix (15); Modified residue (1); Mutagenesis (2); Propeptide (1); Region (3); Sequence conflict (2); Signal peptide (1); Site (2); Turn (2) |
| Keywords | 3D-structure;Carbohydrate metabolism;Cellulose degradation;Direct protein sequencing;Disulfide bond;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Pyrrolidone carboxylic acid;Secreted;Signal |
| Interact With | Itself |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|Ref.3}. |
| Modified Residue | MOD_RES 25; /note=Pyrrolidone carboxylic acid; /evidence=ECO:0000269|Ref.2 |
| Post Translational Modification | PTM: Asn-334 contains mainly a high-mannose-type glycan (Hex(7-9)GlcNAc(2)) in a 3:1 ration with a single GlcNAc. Asn-313 was primarily unglycosylated with a small fraction (18%) bearing a single GlcNAc at this site. {ECO:0000250|UniProtKB:A0A024SH76}. |
| Signal Peptide | SIGNAL 1..18; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (12) |
| Cross Reference PDB | 1CB2; 1HGW; 1HGY; 1QJW; 1QK0; 1QK2; 3CBH; 4AU0; 4AX6; 4AX7; 4I5R; 4I5U; |
| Mapped Pubmed ID | 10508787; 23137336; 23404363; |
| Motif | |
| Gene Encoded By | |
| Mass | 49,653 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=17 uM for cellotriose {ECO:0000269|PubMed:12188666}; KM=2.6 uM for cellotetraose {ECO:0000269|PubMed:12188666}; KM=1.3 uM for cellopentaose {ECO:0000269|PubMed:12188666}; KM=14 uM for cellohexaose {ECO:0000269|PubMed:12188666}; Note=kcat is 0.06 sec(-1) with cellotriose as substrate, 4.1 sec(-1) with cellotetraose as substrate, 1.1 sec(-1) with cellopentaose as substrate and 14 sec(-1) with cellopentaose as substrate. {ECO:0000269|PubMed:12188666}; |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.2.1.176;3.2.1.91; |