IED ID | IndEnz0008000361 |
Enzyme Type ID | cellulase000361 |
Protein Name |
Exoglucanase 2 EC 3.2.1.91 1,4-beta-cellobiohydrolase Cellobiohydrolase 6A Cel6A Exocellobiohydrolase II CBHII Exoglucanase II |
Gene Name | cbh2 |
Organism | Hypocrea jecorina (Trichoderma reesei) |
Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Hypocreomycetidae Hypocreales Hypocreaceae Trichoderma Hypocrea jecorina (Trichoderma reesei) |
Enzyme Sequence | MIVGILTTLATLATLAASVPLEERQACSSVWGQCGGQNWSGPTCCASGSTCVYSNDYYSQCLPGAASSSSSTRAASTTSRVSPTTSRSSSATPPPGSTTTRVPPVGSGTATYSGNPFVGVTPWANAYYASEVSSLAIPSLTGAMATAAAAVAKVPSFMWLDTLDKTPLMEQTLADIRTANKNGGNYAGQFVVYDLPDRDCAALASNGEYSIADGGVAKYKNYIDTIRQIVVEYSDIRTLLVIEPDSLANLVTNLGTPKCANAQSAYLECINYAVTQLNLPNVAMYLDAGHAGWLGWPANQDPAAQLFANVYKNASSPRALRGLATNVANYNGWNITSPPSYTQGNAVYNEKLYIHAIGPLLANHGWSNAFFITDQGRSGKQPTGQQQWGDWCNVIGTGFGIRPSANTGDSLLDSFVWVKPGGECDGTSDSSAPRFDSHCALPDALQPAPQAGAWFQAYFVQLLTNANPSFL |
Enzyme Length | 471 |
Uniprot Accession Number | P07987 |
Absorption | |
Active Site | ACT_SITE 245; /note=Proton donor; /evidence=ECO:0000269|PubMed:12188666 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.; EC=3.2.1.91; Evidence={ECO:0000269|Ref.3}; |
DNA Binding | |
EC Number | 3.2.1.91 |
Enzyme Function | FUNCTION: Exocellobiohydrolases (CBH) that catalyzes the hydrolysis of 1,4-beta-D-glucosidic bonds in cellulose to release the disaccharide cellobiose (Ref.3). The degradation of cellulose involves an interplay between different cellulolytic enzymes. Hydrolysis starts with endoglucanases (EGs), which cut internal beta-1,4-glucosidic bonds in cellulose to reduce the polymerization degree of the substrate and create new chain ends for exocellobiohydrolases (CBHs). The CBHs release the disaccharide cellobiose from the non-reducing end of the cellulose polymer chain. Finally, beta-1,4-glucosidases hydrolyze the cellobiose and other short cello-oligosaccharides into glucose units (Probable). {ECO:0000269|Ref.3, ECO:0000305}. |
Temperature Dependency | |
PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 4-6. {ECO:0000269|PubMed:12188666}; |
Pathway | |
nucleotide Binding | |
Features | Active site (1); Beta strand (14); Chain (1); Disulfide bond (2); Domain (1); Glycosylation (9); Helix (15); Modified residue (1); Mutagenesis (2); Propeptide (1); Region (3); Sequence conflict (2); Signal peptide (1); Site (2); Turn (2) |
Keywords | 3D-structure;Carbohydrate metabolism;Cellulose degradation;Direct protein sequencing;Disulfide bond;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Pyrrolidone carboxylic acid;Secreted;Signal |
Interact With | Itself |
Induction | |
Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|Ref.3}. |
Modified Residue | MOD_RES 25; /note=Pyrrolidone carboxylic acid; /evidence=ECO:0000269|Ref.2 |
Post Translational Modification | PTM: Asn-334 contains mainly a high-mannose-type glycan (Hex(7-9)GlcNAc(2)) in a 3:1 ration with a single GlcNAc. Asn-313 was primarily unglycosylated with a small fraction (18%) bearing a single GlcNAc at this site. {ECO:0000250|UniProtKB:A0A024SH76}. |
Signal Peptide | SIGNAL 1..18; /evidence=ECO:0000255 |
Structure 3D | X-ray crystallography (12) |
Cross Reference PDB | 1CB2; 1HGW; 1HGY; 1QJW; 1QK0; 1QK2; 3CBH; 4AU0; 4AX6; 4AX7; 4I5R; 4I5U; |
Mapped Pubmed ID | 10508787; 23137336; 23404363; |
Motif | |
Gene Encoded By | |
Mass | 49,653 |
Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=17 uM for cellotriose {ECO:0000269|PubMed:12188666}; KM=2.6 uM for cellotetraose {ECO:0000269|PubMed:12188666}; KM=1.3 uM for cellopentaose {ECO:0000269|PubMed:12188666}; KM=14 uM for cellohexaose {ECO:0000269|PubMed:12188666}; Note=kcat is 0.06 sec(-1) with cellotriose as substrate, 4.1 sec(-1) with cellotetraose as substrate, 1.1 sec(-1) with cellopentaose as substrate and 14 sec(-1) with cellopentaose as substrate. {ECO:0000269|PubMed:12188666}; |
Metal Binding | |
Rhea ID | |
Cross Reference Brenda | 3.2.1.176;3.2.1.91; |