Detail Information for IndEnz0008000365
IED ID IndEnz0008000365
Enzyme Type ID cellulase000365
Protein Name Probable glucan endo-1,3-beta-glucosidase BG3
EC 3.2.1.39
Beta-1,3-glucanase 3
AtBG3
Gene Name BG3 At3g57240 F28O9.90
Organism Arabidopsis thaliana (Mouse-ear cress)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress)
Enzyme Sequence MKMCNGSSFLASLPLLLLLLSFILASFFDTAVGQIGVCYGRNGNNLRPASEVVALYQQRNIRRMRLYDPNQETLNALRGSNIELVLDVPNPDLQRLASSQAEADTWVRNNVRNYANVTFRYISVGNEVQPSDQAASFVLPAMQNIERAVSSLGIKVSTAIDTRGISGFPPSSGTFTPEFRSFIAPVISFLSSKQSPLLVNNYPYFSYTGNMRDIRLDYTLFTAPSTVVNDGQNQYRNLFHAILDTVYASLEKAGGGSLEIVVSESGWPTAGGAATGVDNARTYVNNLIQTVKNGSPRRPGRATETYIFAMFDENSKQGPETEKFWGLFLPNLQPKYVVNFN
Enzyme Length 341
Uniprot Accession Number F4J270
Absorption
Active Site ACT_SITE 127; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:O22317; ACT_SITE 264; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:O22317
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.; EC=3.2.1.39; Evidence={ECO:0000305};
DNA Binding
EC Number 3.2.1.39
Enzyme Function FUNCTION: May play a role in plant defense against pathogens. {ECO:0000305|PubMed:23656331}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Erroneous gene model prediction (2); Glycosylation (1); Sequence conflict (1); Signal peptide (1)
Keywords Endoplasmic reticulum;Glycoprotein;Glycosidase;Hydrolase;Reference proteome;Secreted;Signal
Interact With
Induction INDUCTION: By infection with a virulent strain of P.syringae pv. maculicola (PubMed:1824335). Induced by infection with the biotrophic powdery mildew pathogens Golovinomyces cichoracearum and Blumeria graminis (PubMed:16473969). Induced by infection with the turnip vein clearing virus (TVCV) and cucumber mosaic virus (CMV) (PubMed:23656331). {ECO:0000269|PubMed:16473969, ECO:0000269|PubMed:1824335, ECO:0000269|PubMed:23656331}.
Subcellular Location SUBCELLULAR LOCATION: Endoplasmic reticulum {ECO:0000305|PubMed:23656331}. Secreted {ECO:0000250|UniProtKB:P33157}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..25; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 14742872; 14966217; 15047898; 15282545; 15593128; 15763661; 16500996; 16766691; 18538804; 18650403; 28627464; 29777502;
Motif
Gene Encoded By
Mass 37,632
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda