Detail Information for IndEnz0008000367
IED ID IndEnz0008000367
Enzyme Type ID cellulase000367
Protein Name Probable 1,4-beta-D-glucan cellobiohydrolase B
EC 3.2.1.91
Beta-glucancellobiohydrolase B
Exocellobiohydrolase B
Exoglucanase B
Gene Name cbhB ACLA_085260
Organism Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Fumigati Aspergillus clavatus Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107)
Enzyme Sequence MLPSTISYRIYKNALFFAALFGAVQAQKVGTSKAEVHPSMAWQTCAADGTCTTKNGKVVIDANWRWVHDVKGYTNCYTGNTWNAELCPDNESCAENCALEGADYAATYGATTSGNALSLKFVTQSQQKNIGSRLYMMKDDNTYETFKLLNQEFTFDVDVSNLPCGLNGALYFVSMDADGGLSRYTGNEAGAKYGTGYCDSQCPRDLKFINGLANVEGWTPSSSDANAGNGGHGSCCAEMDIWEANSISTAYTPHPCDTPGQAMCNGDSCGGTYSSDRYGGTCDPDGCDFNSYRQGNKSFYGPGMTVDTKKKMTVVTQFLTNDGTATGTLSEIKRFYVQDGKVIANSESTWPNLGGNSLTNDFCKAQKTVFGDMDTFSKHGGMEGMGAALAEGMVLVMSLWDDHNSNMLWLDSNSPTTGTSTTPGVARGSCDISSGDPKDLEANHPDASVVYSNIKVGPIGSTFNSGGSNPGGSTTTTKPATSTTTTKATTTATTNTTGPTGTGVAQPWAQCGGIGYSGPTQCAAPYTCTKQNDYYSQCL
Enzyme Length 539
Uniprot Accession Number A1CU44
Absorption
Active Site ACT_SITE 238; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 243; /note=Proton donor; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.; EC=3.2.1.91;
DNA Binding
EC Number 3.2.1.91
Enzyme Function FUNCTION: The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that cut the disaccharide cellobiose from the non-reducing end of the cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose. {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Disulfide bond (2); Domain (1); Glycosylation (3); Region (3); Signal peptide (1)
Keywords Carbohydrate metabolism;Cellulose degradation;Disulfide bond;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Secreted;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..26; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 57,042
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda