Detail Information for IndEnz0008000368
IED ID IndEnz0008000368
Enzyme Type ID cellulase000368
Protein Name Endoglucanase cel12B
EC 3.2.1.4
Cellulase cel12B
Endo-1,4-beta-glucanase cel12B
Gene Name cel12B MGG_08537
Organism Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Sordariomycetidae Magnaporthales Pyriculariaceae Pyricularia Magnaporthe oryzae (Rice blast fungus) (Pyricularia oryzae) Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae)
Enzyme Sequence MLPRLATVLLSALGASASVLPTTTTRDLASRAAPVASLCTQYAYLKTNGYEILNNLWGIDTATGGGSQCTYYNGAVGPAVAFSSDWTWRGNDNTVKSYVYANRVFERRLVGDIKSLPTSVQWSYNTTNVRANVAYDIFTHTDVNHPNSSGDFELMIWLQRYGGIWPITESSTGSPVERVTIAGYTWELFTGWNGAMRVYSFLPPSGTSYNSFSADVKLFFDYLGQKYAFPASEQYMLIYNFGTEAFTGGPAHFDVSRFQAEVQV
Enzyme Length 264
Uniprot Accession Number G4N5V2
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; Evidence={ECO:0000269|PubMed:20680265};
DNA Binding
EC Number 3.2.1.4
Enzyme Function FUNCTION: Endoglucanase that functions in part to hydrolyze 1,3-1,4-beta-glucan during infection and spore formation. Shows preferential hydrolysis of barley beta-glucan and lichenan, but also hydrolyzes carboxymethylcellulose (CMC) to a slight extent. {ECO:0000269|PubMed:20680265}.
Temperature Dependency BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 30-40 degrees Celsius. {ECO:0000269|PubMed:20680265};
PH Dependency BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 6.0-7.5. {ECO:0000269|PubMed:20680265};
Pathway
nucleotide Binding
Features Chain (1); Glycosylation (2); Signal peptide (1)
Keywords Carbohydrate metabolism;Cellulose degradation;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Secreted;Signal
Interact With
Induction INDUCTION: Expression invreases increased 4-5 days after inoculation, but is not detected during spore formation. {ECO:0000269|PubMed:20680265}.
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..17; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 29,173
Kinetics BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=1.18 mg/ml for barley beta-glucan (when expressed in M.oryzae) {ECO:0000269|PubMed:20680265};
Metal Binding
Rhea ID
Cross Reference Brenda