Detail Information for IndEnz0008000372
IED ID IndEnz0008000372
Enzyme Type ID cellulase000372
Protein Name Probable 1,4-beta-D-glucan cellobiohydrolase A
EC 3.2.1.91
Beta-glucancellobiohydrolase A
Cellobiohydrolase D
Exocellobiohydrolase A
Exoglucanase A
Gene Name cbhA celD NFIA_052720
Organism Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) (Aspergillus fischerianus)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Fumigati Aspergillus fischeri Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) (Aspergillus fischerianus)
Enzyme Sequence MHQRALLFSALAVAANAQQVGTQKPETHPPLTWQKCTAAGSCSQQSGSVVIDANWRWLHSTKDTTNCYTGNTWNTELCPDNESCAQNCAVDGADYAGTYGVTTSGSELKLSFVTGANVGSRLYLMQDDETYQHFNLLNNEFTFDVDVSNLPCGLNGALYFVAMDADGGMSKYPSNKAGAKYGTGYCDSQCPRDLKFINGMANVEGWKPSSNDKNAGVGGHGSCCPEMDIWEANSISTAVTPHPCDDVSQTMCSGDACGGTYSATRYAGTCDPDGCDFNPFRMGNESFYGPGKIVDTKSEMTVVTQFITADGTDTGALSEIKRLYVQNGKVIANSVSNVADVSGNSISSDFCTAQKKAFGDEDIFAKHGGLSGMGKALSEMVLIMSIWDDHHSSMMWLDSTYPTDADPSKPGVARGTCEHGAGDPEKVESQHPDASVTFSNIKFGPIGSTYKA
Enzyme Length 452
Uniprot Accession Number A1DMA5
Absorption
Active Site ACT_SITE 226; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 231; /note=Proton donor; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.; EC=3.2.1.91;
DNA Binding
EC Number 3.2.1.91
Enzyme Function FUNCTION: The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that cut the disaccharide cellobiose from the non-reducing end of the cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose. {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Glycosylation (2); Region (1); Signal peptide (1)
Keywords Carbohydrate metabolism;Cellulose degradation;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Secreted;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..17; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 48,193
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda